Characterization of rhamnolipids produced by non-pathogenic Acinetobacter and Enterobacter bacteria

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F13%3A00395777" target="_blank" >RIV/61388971:_____/13:00395777 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22340/13:43894966 RIV/60461373:22330/13:43894966 RIV/60461373:22810/13:43894966

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.biortech.2012.12.085" target="_blank" >http://dx.doi.org/10.1016/j.biortech.2012.12.085</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.biortech.2012.12.085" target="_blank" >10.1016/j.biortech.2012.12.085</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Characterization of rhamnolipids produced by non-pathogenic Acinetobacter and Enterobacter bacteria

Popis výsledku v původním jazyce

Rhamnolipids were separated from supernatant and further purified by thin-layer chromatography. Mass spectrometry with negative electrospray ionization revealed rhamnolipid homologues varying in chain length and unsaturation. Tandem mass spectrometry identified mono-rhamnolipid and dirhamnolipid homologues containing one or two 3-hydroxy fatty acids. Several media differing in carbon (sunflower oil, glycerol and sodium citrate), nitrogen (ammonium ions, nitrate) and phosphorus (total content) source, respectively, were tested to obtain enhanced rhamnolipid production. The best production (0.56 g/l) was obtained when nitrate was used as a nitrogen source. Both strains produced rhamnolipids that exhibited excellent emulsification activity with aromatic and aliphatic hydrocarbons and several plant oils. Unlike P. aeruginosa the two strains, i.e. Acinetobacter and Enterobacter, are not pathogenic to humans

Název v anglickém jazyce

Characterization of rhamnolipids produced by non-pathogenic Acinetobacter and Enterobacter bacteria

Popis výsledku anglicky

Rhamnolipids were separated from supernatant and further purified by thin-layer chromatography. Mass spectrometry with negative electrospray ionization revealed rhamnolipid homologues varying in chain length and unsaturation. Tandem mass spectrometry identified mono-rhamnolipid and dirhamnolipid homologues containing one or two 3-hydroxy fatty acids. Several media differing in carbon (sunflower oil, glycerol and sodium citrate), nitrogen (ammonium ions, nitrate) and phosphorus (total content) source, respectively, were tested to obtain enhanced rhamnolipid production. The best production (0.56 g/l) was obtained when nitrate was used as a nitrogen source. Both strains produced rhamnolipids that exhibited excellent emulsification activity with aromatic and aliphatic hydrocarbons and several plant oils. Unlike P. aeruginosa the two strains, i.e. Acinetobacter and Enterobacter, are not pathogenic to humans

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/GAP503%2F11%2F0215" target="_blank" >GAP503/11/0215: Analýza složení triacylglycerolů a dalších lipidů v mikrobiálních olejích a tucích důležitých pro výživu.</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Bioresource Technology

ISSN

0960-8524

e-ISSN

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Svazek periodika

130

Číslo periodika v rámci svazku

FEB 2013

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

510-516

Kód UT WoS článku

000316032300068

EID výsledku v databázi Scopus

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