Identification of regioisomers and enantiomers of triacylglycerols in different yeasts using reversed- and chiral-phase LC?MS

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F13%3A00397491" target="_blank" >RIV/61388971:_____/13:00397491 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1002/jssc.201300657" target="_blank" >http://dx.doi.org/10.1002/jssc.201300657</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jssc.201300657" target="_blank" >10.1002/jssc.201300657</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Identification of regioisomers and enantiomers of triacylglycerols in different yeasts using reversed- and chiral-phase LC?MS

Popis výsledku v původním jazyce

LC with atmospheric pressure chemical ionization (ACPI) MS with RP and chiral phase was used for separation of triacylglycerols (TAGs) from yeasts of the genera Candida, Kluyveromyces, Rhodotorula, Saccharomyces, Torulospora, Trichosporon, and Yarrowia.Chiral LC?APCI-MS is based on using two columns in series packed with a 3,5-dimethylphenyl carbamatemodified _-cyclodextrin chiral phase. All regioisomers and enantiomers of TAGs containing one to five double bonds were separated.Molecular species of TAGs, i.e. regioisomers and enantiomers, were identified and quantified by MS/MS. Among the 94 identified TAGs, the most abundant were triolein, oleopalmitoleoolein, and dipalmitoleoolein. In strains producing palmitoleic acid in amounts >25% of total fattyacids (FAs), this acid, or unsaturated FA is bound in sn-1. In strains containing palmitoleic acid at 10?25% total FAs this acid is mainly bound in sn-3, saturated FA being bound in sn-1. Strains containing <10% palmitoleic acid form pre

Název v anglickém jazyce

Identification of regioisomers and enantiomers of triacylglycerols in different yeasts using reversed- and chiral-phase LC?MS

Popis výsledku anglicky

LC with atmospheric pressure chemical ionization (ACPI) MS with RP and chiral phase was used for separation of triacylglycerols (TAGs) from yeasts of the genera Candida, Kluyveromyces, Rhodotorula, Saccharomyces, Torulospora, Trichosporon, and Yarrowia.Chiral LC?APCI-MS is based on using two columns in series packed with a 3,5-dimethylphenyl carbamatemodified _-cyclodextrin chiral phase. All regioisomers and enantiomers of TAGs containing one to five double bonds were separated.Molecular species of TAGs, i.e. regioisomers and enantiomers, were identified and quantified by MS/MS. Among the 94 identified TAGs, the most abundant were triolein, oleopalmitoleoolein, and dipalmitoleoolein. In strains producing palmitoleic acid in amounts >25% of total fattyacids (FAs), this acid, or unsaturated FA is bound in sn-1. In strains containing palmitoleic acid at 10?25% total FAs this acid is mainly bound in sn-3, saturated FA being bound in sn-1. Strains containing <10% palmitoleic acid form pre

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/GAP503%2F11%2F0215" target="_blank" >GAP503/11/0215: Analýza složení triacylglycerolů a dalších lipidů v mikrobiálních olejích a tucích důležitých pro výživu.</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Separation Science

ISSN

1615-9306

e-ISSN

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Svazek periodika

36

Číslo periodika v rámci svazku

20

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

11

Strana od-do

3310-3320

Kód UT WoS článku

000325482500002

EID výsledku v databázi Scopus

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