Biodegradation of phenol using recombinant plasmid-carrying Rhodococcus erythropolis strains

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F13%3A00426259" target="_blank" >RIV/61388971:_____/13:00426259 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.ibiod.2012.05.017" target="_blank" >http://dx.doi.org/10.1016/j.ibiod.2012.05.017</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ibiod.2012.05.017" target="_blank" >10.1016/j.ibiod.2012.05.017</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Biodegradation of phenol using recombinant plasmid-carrying Rhodococcus erythropolis strains

Popis výsledku v původním jazyce

The aim of the study was to utilize current knowledge of the phenol catabolic genes in Rhodococcus erythropolis CCM2595 to construct improved phenol degraders and test their bioremediation potential using real industrial wastewater. The genes pheA2A1-pheR-catR-catABC, coding for enzymes of the phenol degradation pathway and transcriptional regulators in R. erythropolis CCM2595, were cloned in various combinations in the multicopy R. erythropolis plasmid vector pSRK21 and the resulting constructs were introduced into R. erythropolis cells. All plasmid-carrying strains showed increased phenol hydroxylase activity. The strains harboring constructs pSRKAphe-cat and pSRKBphe-cat (both carrying all enzyme-coding genes of the phenol catabolic gene cluster) exhibited the highest phenol hydroxylase activities and proved to be the most efficient phenol degraders in minimal medium with 0.3 g L-1 phenol. The recombinant strains were up to 50% more efficient than the wild-type strain in the bioreme

Název v anglickém jazyce

Biodegradation of phenol using recombinant plasmid-carrying Rhodococcus erythropolis strains

Popis výsledku anglicky

The aim of the study was to utilize current knowledge of the phenol catabolic genes in Rhodococcus erythropolis CCM2595 to construct improved phenol degraders and test their bioremediation potential using real industrial wastewater. The genes pheA2A1-pheR-catR-catABC, coding for enzymes of the phenol degradation pathway and transcriptional regulators in R. erythropolis CCM2595, were cloned in various combinations in the multicopy R. erythropolis plasmid vector pSRK21 and the resulting constructs were introduced into R. erythropolis cells. All plasmid-carrying strains showed increased phenol hydroxylase activity. The strains harboring constructs pSRKAphe-cat and pSRKBphe-cat (both carrying all enzyme-coding genes of the phenol catabolic gene cluster) exhibited the highest phenol hydroxylase activities and proved to be the most efficient phenol degraders in minimal medium with 0.3 g L-1 phenol. The recombinant strains were up to 50% more efficient than the wild-type strain in the bioreme

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/2B08062" target="_blank" >2B08062: Genetické a fyziologické manipulace s bakteriálními degradéry aromatických polutantů a jejich využití</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Biodeterioration & Biodegradation

ISSN

0964-8305

e-ISSN

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Svazek periodika

84

Číslo periodika v rámci svazku

OCT 2013

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

6

Strana od-do

179-184

Kód UT WoS článku

000322942000025

EID výsledku v databázi Scopus

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