Induction and carbon catabolite repression of phenol degradation genes in Rhodococcus erythropolis nad Rhodococcus jostii

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F14%3A00433025" target="_blank" >RIV/61388971:_____/14:00433025 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s00253-014-5881-6" target="_blank" >http://dx.doi.org/10.1007/s00253-014-5881-6</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00253-014-5881-6" target="_blank" >10.1007/s00253-014-5881-6</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Induction and carbon catabolite repression of phenol degradation genes in Rhodococcus erythropolis nad Rhodococcus jostii

Popis výsledku v původním jazyce

Rhodococcus erythropolis CCM2595 is able to efficiently utilize phenol and other aromatic compounds. We cloned and sequenced its complete gene cluster - catA, catB, catC, catR, pheR, pheA2, pheA1 - involved in the ortho-cleavage pathway of phenol. The activity of the key enzyme of the phenol degradation pathway, two-component phenol hydroxylase, was found to be induced by phenol. When both phenol and succinate were present in the medium, phenol hydroxylase activity decreased substantially. To analyze the regulation of phenol degradation at the transcriptional level, the transcriptional fusions of the divergently oriented promoters PpheA2 and PpheR with the gfpuv reporter gene were constructed. The promoters driving expression of the genes of the pheR-pheA2pheA1 cluster were localized by determining the respective transcriptional start points

Název v anglickém jazyce

Induction and carbon catabolite repression of phenol degradation genes in Rhodococcus erythropolis nad Rhodococcus jostii

Popis výsledku anglicky

Rhodococcus erythropolis CCM2595 is able to efficiently utilize phenol and other aromatic compounds. We cloned and sequenced its complete gene cluster - catA, catB, catC, catR, pheR, pheA2, pheA1 - involved in the ortho-cleavage pathway of phenol. The activity of the key enzyme of the phenol degradation pathway, two-component phenol hydroxylase, was found to be induced by phenol. When both phenol and succinate were present in the medium, phenol hydroxylase activity decreased substantially. To analyze the regulation of phenol degradation at the transcriptional level, the transcriptional fusions of the divergently oriented promoters PpheA2 and PpheR with the gfpuv reporter gene were constructed. The promoters driving expression of the genes of the pheR-pheA2pheA1 cluster were localized by determining the respective transcriptional start points

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Applied Microbiology and Biotechnology

ISSN

0175-7598

e-ISSN

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Svazek periodika

98

Číslo periodika v rámci svazku

19

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

12

Strana od-do

8267-8279

Kód UT WoS článku

000342072500018

EID výsledku v databázi Scopus

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