Chemoenzymatic Synthesis of beta-D-Glucosides using Cellobiose Phosphorylase from Clostridium thermocellum

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F15%3A00445823" target="_blank" >RIV/61388971:_____/15:00445823 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1002/adsc.201500077" target="_blank" >http://dx.doi.org/10.1002/adsc.201500077</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/adsc.201500077" target="_blank" >10.1002/adsc.201500077</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Chemoenzymatic Synthesis of beta-D-Glucosides using Cellobiose Phosphorylase from Clostridium thermocellum

Popis výsledku v původním jazyce

Over the past decade, disaccharide phosphorylases have been successfully applied for the synthesis of numerous a-glucosides. In contrast, much less research has been done with respect to the production of beta-glucosides. Although cellobiose phosphorylase was already successfully used for the synthesis of various disaccharides and branched trisaccharides, its glycosylation potential towards small organic compounds has not been explored to date. Unfortunately, disaccharide phosphorylases typically have avery low affinity for non-carbohydrate acceptors, which urges the addition of solvents. The ionic liquid AMMOENG (TM) 101 and ethyl acetate were identified as the most promising solvents, allowing the synthesis of various beta-glucosides. Next to hexyl,heptyl, octyl, nonyl, decyl and undecyl beta-D-glucopyranosides, also the formation of vanillyl 4-O-beta-D-glucopyranoside, 2-phenylethyl beta-D-glucopyranoside, beta-citronellyl beta-D-glucopyranoside and 1-O-beta-D-glucopyranosyl hydro

Název v anglickém jazyce

Chemoenzymatic Synthesis of beta-D-Glucosides using Cellobiose Phosphorylase from Clostridium thermocellum

Popis výsledku anglicky

Over the past decade, disaccharide phosphorylases have been successfully applied for the synthesis of numerous a-glucosides. In contrast, much less research has been done with respect to the production of beta-glucosides. Although cellobiose phosphorylase was already successfully used for the synthesis of various disaccharides and branched trisaccharides, its glycosylation potential towards small organic compounds has not been explored to date. Unfortunately, disaccharide phosphorylases typically have avery low affinity for non-carbohydrate acceptors, which urges the addition of solvents. The ionic liquid AMMOENG (TM) 101 and ethyl acetate were identified as the most promising solvents, allowing the synthesis of various beta-glucosides. Next to hexyl,heptyl, octyl, nonyl, decyl and undecyl beta-D-glucopyranosides, also the formation of vanillyl 4-O-beta-D-glucopyranoside, 2-phenylethyl beta-D-glucopyranoside, beta-citronellyl beta-D-glucopyranoside and 1-O-beta-D-glucopyranosyl hydro

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Advanced Synthesis & Catalysis

ISSN

1615-4150

e-ISSN

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Svazek periodika

357

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

9

Strana od-do

1961-1969

Kód UT WoS článku

000355235700035

EID výsledku v databázi Scopus

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