The Arabidopsis mitogen-activated protein kinase 6 is associated with ?-tubulin on microtubules, phosphorylates EB1c and maintains spindle orientation under nitrosative stress

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F15%3A00447446" target="_blank" >RIV/61388971:_____/15:00447446 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1111/nph.13501" target="_blank" >http://dx.doi.org/10.1111/nph.13501</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/nph.13501" target="_blank" >10.1111/nph.13501</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The Arabidopsis mitogen-activated protein kinase 6 is associated with ?-tubulin on microtubules, phosphorylates EB1c and maintains spindle orientation under nitrosative stress

Popis výsledku v původním jazyce

Stress-activated plant mitogen-activated protein (MAP) kinase pathways play roles in growth adaptation to the environment by modulating cell division through cytoskeletal regulation, but the mechanisms are poorly understood. We performed protein interaction and phosphorylation experiments with cytoskeletal proteins, mass spectrometric identification of MPK6 complexes and immunofluorescence analyses of the microtubular cytoskeleton of mitotic cells using wild-type, mpk6-2 mutant and plants overexpressingthe MAP kinase-inactivating phosphatase, AP2C3. We showed that MPK6 interacted with -tubulin and co-sedimented with plant microtubules polymerized invitro. It was the active form of MAP kinase that was enriched with microtubules and followed similar dynamics to -tubulin, moving from poles to midzone during the anaphase-to-telophase transition. We found a novel substrate for MPK6, the microtubule plus end protein, EB1c. The mpk6-2 mutant was sensitive to 3-nitro-l-tyrosine (NO2-Tyr) trea

Název v anglickém jazyce

The Arabidopsis mitogen-activated protein kinase 6 is associated with ?-tubulin on microtubules, phosphorylates EB1c and maintains spindle orientation under nitrosative stress

Popis výsledku anglicky

Stress-activated plant mitogen-activated protein (MAP) kinase pathways play roles in growth adaptation to the environment by modulating cell division through cytoskeletal regulation, but the mechanisms are poorly understood. We performed protein interaction and phosphorylation experiments with cytoskeletal proteins, mass spectrometric identification of MPK6 complexes and immunofluorescence analyses of the microtubular cytoskeleton of mitotic cells using wild-type, mpk6-2 mutant and plants overexpressingthe MAP kinase-inactivating phosphatase, AP2C3. We showed that MPK6 interacted with -tubulin and co-sedimented with plant microtubules polymerized invitro. It was the active form of MAP kinase that was enriched with microtubules and followed similar dynamics to -tubulin, moving from poles to midzone during the anaphase-to-telophase transition. We found a novel substrate for MPK6, the microtubule plus end protein, EB1c. The mpk6-2 mutant was sensitive to 3-nitro-l-tyrosine (NO2-Tyr) trea

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

New Phytologist

ISSN

0028-646X

e-ISSN

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Svazek periodika

207

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

14

Strana od-do

1061-1074

Kód UT WoS článku

000358965800016

EID výsledku v databázi Scopus

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