Differentially expressed proteins in human MCF-7 breast cancer cells sensitive and resistant to paclitaxel

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F15%3A00451983" target="_blank" >RIV/61388971:_____/15:00451983 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11120/15:43909252

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.yexcr.2014.12.005" target="_blank" >http://dx.doi.org/10.1016/j.yexcr.2014.12.005</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.yexcr.2014.12.005" target="_blank" >10.1016/j.yexcr.2014.12.005</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Differentially expressed proteins in human MCF-7 breast cancer cells sensitive and resistant to paclitaxel

Popis výsledku v původním jazyce

Resistance of cancer cells to chemotherapeutic agents is one of the main causes of treatment failure. In order to detect proteins potentially involved in the mechanism of resistance to taxanes, we assessed differences in protein expression in MCF-7 breast cancer cells that are sensitive to paclitaxel and in the same cells with acquired resistance to paclitaxel (established in our lab). Proteins were separated using two-dimensional electrophoresis. Changes in their expression were determined and proteinswith altered expression were identified using mass spectrometry. Changes in their expression were confirmed using western blot analysis. With these techniques, we found three proteins expressed differently in resistant MCF-7 cells, i.e., thyroid hormone-interacting protein 6 (TRIP6; upregulated to 650%), heat shock protein 27 (HSP27; downregulated to 50%) and cathepsin D (downregulated to 28%). Silencing of TRIP6 expression by specific siRNA leads to decreased number of grown resistant

Název v anglickém jazyce

Differentially expressed proteins in human MCF-7 breast cancer cells sensitive and resistant to paclitaxel

Popis výsledku anglicky

Resistance of cancer cells to chemotherapeutic agents is one of the main causes of treatment failure. In order to detect proteins potentially involved in the mechanism of resistance to taxanes, we assessed differences in protein expression in MCF-7 breast cancer cells that are sensitive to paclitaxel and in the same cells with acquired resistance to paclitaxel (established in our lab). Proteins were separated using two-dimensional electrophoresis. Changes in their expression were determined and proteinswith altered expression were identified using mass spectrometry. Changes in their expression were confirmed using western blot analysis. With these techniques, we found three proteins expressed differently in resistant MCF-7 cells, i.e., thyroid hormone-interacting protein 6 (TRIP6; upregulated to 650%), heat shock protein 27 (HSP27; downregulated to 50%) and cathepsin D (downregulated to 28%). Silencing of TRIP6 expression by specific siRNA leads to decreased number of grown resistant

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/NT13679" target="_blank" >NT13679: Studie mechanismu účinků biomarkerů korelujících s výsledkem léčby karcinomu prsu</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Experimental Cell Research

ISSN

0014-4827

e-ISSN

—

Svazek periodika

333

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

1-10

Kód UT WoS článku

000352823600001

EID výsledku v databázi Scopus

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