An in-depth analysis of actinobacterial communities shows their high diversity in grassland soils along a gradient of mixed heavy metal contamination

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F15%3A00455444" target="_blank" >RIV/61388971:_____/15:00455444 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s00374-015-1029-9" target="_blank" >http://dx.doi.org/10.1007/s00374-015-1029-9</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00374-015-1029-9" target="_blank" >10.1007/s00374-015-1029-9</a>

Jazyk výsledku

angličtina

Název v původním jazyce

An in-depth analysis of actinobacterial communities shows their high diversity in grassland soils along a gradient of mixed heavy metal contamination

Popis výsledku v původním jazyce

Several previous studies indicated that Actinobacteria may be enriched in soils with elevated content of heavy metals. In this study, we have developed a method for the in-depth analysis of actinobacterial communities in soil through phylum-targeted high-throughput sequencing and used it to address this question and examine the community composition in grassland soils along a gradient of heavy metal contamination (Cu, Zn, Cd, Pb). The use of the 16Sact111r primer specific for Actinobacteria resulted ina dataset obtained by pyrosequencing where over 98 % of the sequences belonged to Actinobacteria. The diversity within the Actinobacterial community was not affected by the heavy metals, but the contamination was the most important factor affecting community composition. The most significant changes in community composition were due to the content of Cu and Pb, while the effects of Zn and Cd were relatively minor. For the most abundant actinobacterial taxa, the abundance of taxa identifi

Název v anglickém jazyce

An in-depth analysis of actinobacterial communities shows their high diversity in grassland soils along a gradient of mixed heavy metal contamination

Popis výsledku anglicky

Several previous studies indicated that Actinobacteria may be enriched in soils with elevated content of heavy metals. In this study, we have developed a method for the in-depth analysis of actinobacterial communities in soil through phylum-targeted high-throughput sequencing and used it to address this question and examine the community composition in grassland soils along a gradient of heavy metal contamination (Cu, Zn, Cd, Pb). The use of the 16Sact111r primer specific for Actinobacteria resulted ina dataset obtained by pyrosequencing where over 98 % of the sequences belonged to Actinobacteria. The diversity within the Actinobacterial community was not affected by the heavy metals, but the contamination was the most important factor affecting community composition. The most significant changes in community composition were due to the content of Cu and Pb, while the effects of Zn and Cd were relatively minor. For the most abundant actinobacterial taxa, the abundance of taxa identifi

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biology and Fertility of Soils

ISSN

0178-2762

e-ISSN

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Svazek periodika

51

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

11

Strana od-do

827-837

Kód UT WoS článku

000361348100006

EID výsledku v databázi Scopus

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