Target antigens for Hs-14 monoclonal antibody and their various expression in normozoospermic and asthenozoospermic men

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F15%3A00455664" target="_blank" >RIV/61388971:_____/15:00455664 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/86652036:_____/15:00455664

Výsledek na webu

<a href="http://link.springer.com/article/10.1186/s12610-015-0025-0" target="_blank" >http://link.springer.com/article/10.1186/s12610-015-0025-0</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s12610-015-0025-0" target="_blank" >10.1186/s12610-015-0025-0</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Target antigens for Hs-14 monoclonal antibody and their various expression in normozoospermic and asthenozoospermic men

Popis výsledku v původním jazyce

Poor semen quality is one of the main causes of infertility. We have generated a set of monoclonal antibodies to human sperm and used them to investigate sperm quality. Some of these antibodies found differences in the expression of proteins between normal sperm and pathological sperm displaying severe defects. One of them was the Hs-14 antibody. The aim of this paper was to determine the target protein of the Hs-14 monoclonal antibody and to investigate the expression of the Hs-14-reacting protein on the sperm of asthenozoospermic men with sperm motility defect and of healthy normozoospermic men. The Hs-14 antibody binds fibronectin, ?-tubulin and valosin-containing protein - new name for this protein is transitional endoplasmic reticulum ATPase (TERA). Since the Hs-14 reaction with TERA remained the strongest at the highest antibody dilution, and Hs-14 consistently labelled the same spot or band as the monospecific anti-TERA antibody on immunoblots, we assume that TERA is an Hs-14-sp

Název v anglickém jazyce

Target antigens for Hs-14 monoclonal antibody and their various expression in normozoospermic and asthenozoospermic men

Popis výsledku anglicky

Poor semen quality is one of the main causes of infertility. We have generated a set of monoclonal antibodies to human sperm and used them to investigate sperm quality. Some of these antibodies found differences in the expression of proteins between normal sperm and pathological sperm displaying severe defects. One of them was the Hs-14 antibody. The aim of this paper was to determine the target protein of the Hs-14 monoclonal antibody and to investigate the expression of the Hs-14-reacting protein on the sperm of asthenozoospermic men with sperm motility defect and of healthy normozoospermic men. The Hs-14 antibody binds fibronectin, ?-tubulin and valosin-containing protein - new name for this protein is transitional endoplasmic reticulum ATPase (TERA). Since the Hs-14 reaction with TERA remained the strongest at the highest antibody dilution, and Hs-14 consistently labelled the same spot or band as the monospecific anti-TERA antibody on immunoblots, we assume that TERA is an Hs-14-sp

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Basic and Clinical Andrology

ISSN

2051-4190

e-ISSN

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Svazek periodika

25

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

12

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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