Evaluation of the expression of sperm proteins in normozoospermic and asthezoospermic sperm samples using monoclonal antibodies, flow cytometry and fluorescence microscopy

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F15%3A00445182" target="_blank" >RIV/86652036:_____/15:00445182 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Evaluation of the expression of sperm proteins in normozoospermic and asthezoospermic sperm samples using monoclonal antibodies, flow cytometry and fluorescence microscopy

Popis výsledku v původním jazyce

Asthenozoospermia is one of the main seminal pathologies underlying male infertility. In our study we aimed to evaluate the ability of specific antibodies to detect the differential expression of selected protein markers by fluorescent microscopy and flow cytometry techniques. Therefore we analyzed sperm samples from 30 men with normal spermiograms and 30 men with asthenozoospermia (<40% motile spermatozoa) by the panel of our diagnostic anti-human sperm (Hs) antibodies. These antibodies were prepared in our laboratory and some of them are used in clinical practice as a tool for the differential diagnosis of various sperm pathologies. Both fluorescent microscopy and flow cytometry analysis revealed quantitative differences in the protein abundances between normozoospermic and asthenozoospermic sperm samples, namely, in GAPDHS, evaluated with Hs-8 MoAb, VCP (valosin-containing protein), evaluated with Hs-14 MoAb, and ATP synthase (cAMP-dependent protein kinase II, PRKAR2A), evaluated wi

Název v anglickém jazyce

Evaluation of the expression of sperm proteins in normozoospermic and asthezoospermic sperm samples using monoclonal antibodies, flow cytometry and fluorescence microscopy

Popis výsledku anglicky

Asthenozoospermia is one of the main seminal pathologies underlying male infertility. In our study we aimed to evaluate the ability of specific antibodies to detect the differential expression of selected protein markers by fluorescent microscopy and flow cytometry techniques. Therefore we analyzed sperm samples from 30 men with normal spermiograms and 30 men with asthenozoospermia (<40% motile spermatozoa) by the panel of our diagnostic anti-human sperm (Hs) antibodies. These antibodies were prepared in our laboratory and some of them are used in clinical practice as a tool for the differential diagnosis of various sperm pathologies. Both fluorescent microscopy and flow cytometry analysis revealed quantitative differences in the protein abundances between normozoospermic and asthenozoospermic sperm samples, namely, in GAPDHS, evaluated with Hs-8 MoAb, VCP (valosin-containing protein), evaluated with Hs-14 MoAb, and ATP synthase (cAMP-dependent protein kinase II, PRKAR2A), evaluated wi

Druh

O - Ostatní výsledky

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů