Characterization of human sperm protein recognized by monoclonal antibody HS-8

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Characterization of human sperm protein recognized by monoclonal antibody HS-8

Popis výsledku v původním jazyce

Problem: Characterization of the sperm protein detected by monoclonal antibody (MoAb) Hs-8, which was prepared in our laboratory by immunization of BALB/c mice with human ejaculated sperms. Method of Study: Indirect immunofluorescence, electrophoresis, Western blotting and protein sequencing were used to characterize this protein. For detailed analysis, commercial mouse monoclonal antibody against human sperm-specific glycolytic enzyme (GAPDHS ? ab57062, Abcam, UK) was used. Results: MoAb Hs-8 is a human sperm antibody that cross-reacts with the Hs-8-related protein in spermatozoa of other mammalian species (boar, mouse). In the immunofluorescence test Hs-8 antibody reacted with a protein localised in the arcosomal part of the sperm head and in the principal piece of the sperm flagellum. MoAb Hs-8 labelled a protein of 44 kDa in immunoblot test in the extract of human sperm. Sequence analysis characterized protein Hs-8 as GAPDS (glyceraldehyde 3-phosphate dehydrohenase-spermatogenic).

Název v anglickém jazyce

Characterization of human sperm protein recognized by monoclonal antibody HS-8

Popis výsledku anglicky

Problem: Characterization of the sperm protein detected by monoclonal antibody (MoAb) Hs-8, which was prepared in our laboratory by immunization of BALB/c mice with human ejaculated sperms. Method of Study: Indirect immunofluorescence, electrophoresis, Western blotting and protein sequencing were used to characterize this protein. For detailed analysis, commercial mouse monoclonal antibody against human sperm-specific glycolytic enzyme (GAPDHS ? ab57062, Abcam, UK) was used. Results: MoAb Hs-8 is a human sperm antibody that cross-reacts with the Hs-8-related protein in spermatozoa of other mammalian species (boar, mouse). In the immunofluorescence test Hs-8 antibody reacted with a protein localised in the arcosomal part of the sperm head and in the principal piece of the sperm flagellum. MoAb Hs-8 labelled a protein of 44 kDa in immunoblot test in the extract of human sperm. Sequence analysis characterized protein Hs-8 as GAPDS (glyceraldehyde 3-phosphate dehydrohenase-spermatogenic).

Druh

O - Ostatní výsledky

CEP obor

EC - Imunologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů