Human seminal plasma proteins on sperm surface detected by monoclonal antibodies

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F09%3A00326897" target="_blank" >RIV/86652036:_____/09:00326897 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Human seminal plasma proteins on sperm surface detected by monoclonal antibodies

Popis výsledku v původním jazyce

We have generated antibodies to sperm and in our panel of mouse monoclonal antibodies to human spermatozoa several antibodies - Hs-3, Hs-16, Hs-23, Hs-9, Hs-31, Hs-32 and Hs-37 - reacted with human seminal plasma. These antibodies were characterized in detail using indirect immunofluorescence, Western blot techniques and MALDI TOF sequence analysis and recognized apolipoprotein J (clusterin), semenogelin I, secretory actin-binding protein (SABP) and enolase I. Proteins detected by our monoclonal antibodies can be useful in investigation of the mechanisms involved in sperm pathology and also represent candidates for diagnostic markers during sperm quality assessement. Further experiments concerning the role of above mentioned proteins in capacitation, acrosomal reaction and sperm-egg binding are in progress.

Název v anglickém jazyce

Human seminal plasma proteins on sperm surface detected by monoclonal antibodies

Popis výsledku anglicky

We have generated antibodies to sperm and in our panel of mouse monoclonal antibodies to human spermatozoa several antibodies - Hs-3, Hs-16, Hs-23, Hs-9, Hs-31, Hs-32 and Hs-37 - reacted with human seminal plasma. These antibodies were characterized in detail using indirect immunofluorescence, Western blot techniques and MALDI TOF sequence analysis and recognized apolipoprotein J (clusterin), semenogelin I, secretory actin-binding protein (SABP) and enolase I. Proteins detected by our monoclonal antibodies can be useful in investigation of the mechanisms involved in sperm pathology and also represent candidates for diagnostic markers during sperm quality assessement. Further experiments concerning the role of above mentioned proteins in capacitation, acrosomal reaction and sperm-egg binding are in progress.

Druh

O - Ostatní výsledky

CEP obor

DN - Vliv životního prostředí na zdraví

OECD FORD obor

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Projekt

<a href="/cs/project/1M06011" target="_blank" >1M06011: Centrum molekulárních metod monitorování difúzního znečištění životního prostředí</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů