Use of In Vitro Transcription System for Analysis of Corynebacterium glutamicum Promoters Recognized by Two Sigma Factors

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F16%3A00462752" target="_blank" >RIV/61388971:_____/16:00462752 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s00284-016-1077-x" target="_blank" >http://dx.doi.org/10.1007/s00284-016-1077-x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00284-016-1077-x" target="_blank" >10.1007/s00284-016-1077-x</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Use of In Vitro Transcription System for Analysis of Corynebacterium glutamicum Promoters Recognized by Two Sigma Factors

Popis výsledku v původním jazyce

Promoter activities in Corynebacterium glutamicum strains with deletions of genes encoding sigma factors of RNA polymerase suggested that transcription from some promoters is controlled by two sigma factors. To prove that different sigma factors are involved in the recognition of selected Corynebacterium glutamicum promoters, in vitro transcription system was applied. It was found that a typical housekeeping promoter Pper interacts with the alternative sigma factor sigma(B) in addition to the primary sigma factor sigma(A). On the other way round, the sigma(B)-dependent promoter of the pqo gene that is expressed mainly in the stationary growth phase was active also with sigma(A). Some promoters of genes involved in stress responses (P1clgR, P2dnaK, and P2dnaJ2) were found to be recognized by two stress-responding sigma factors, sigma(H) and sigma(E).

Název v anglickém jazyce

Use of In Vitro Transcription System for Analysis of Corynebacterium glutamicum Promoters Recognized by Two Sigma Factors

Popis výsledku anglicky

Promoter activities in Corynebacterium glutamicum strains with deletions of genes encoding sigma factors of RNA polymerase suggested that transcription from some promoters is controlled by two sigma factors. To prove that different sigma factors are involved in the recognition of selected Corynebacterium glutamicum promoters, in vitro transcription system was applied. It was found that a typical housekeeping promoter Pper interacts with the alternative sigma factor sigma(B) in addition to the primary sigma factor sigma(A). On the other way round, the sigma(B)-dependent promoter of the pqo gene that is expressed mainly in the stationary growth phase was active also with sigma(A). Some promoters of genes involved in stress responses (P1clgR, P2dnaK, and P2dnaJ2) were found to be recognized by two stress-responding sigma factors, sigma(H) and sigma(E).

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

—

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Current Microbiology

ISSN

0343-8651

e-ISSN

—

Svazek periodika

73

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

401-408

Kód UT WoS článku

000381114200015

EID výsledku v databázi Scopus

2-s2.0-84982792270