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Assignment of sigma factors of RNA polymerase to promoters in Corynebacterium glutamicum

Identifikátory výsledku

  • Kód výsledku v IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F17%3A00476299" target="_blank" >RIV/61388971:_____/17:00476299 - isvavai.cz</a>

  • Výsledek na webu

    <a href="http://dx.doi.org/10.1186/s13568-017-0436-8" target="_blank" >http://dx.doi.org/10.1186/s13568-017-0436-8</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1186/s13568-017-0436-8" target="_blank" >10.1186/s13568-017-0436-8</a>

Alternativní jazyky

  • Jazyk výsledku

    angličtina

  • Název v původním jazyce

    Assignment of sigma factors of RNA polymerase to promoters in Corynebacterium glutamicum

  • Popis výsledku v původním jazyce

    Corynebacterium glutamicum is an important industrial producer of various amino acids and other metabolites. The C. glutamicum genome encodes seven sigma subunits (factors) of RNA polymerase: the primary sigma factor SigA (sA), the primary-like sigma(B) and five alternative sigma factors (sigma(C), sigma(D), sigma(E), sigma(H) and sigma(M)). We have developed in vitro and in vivo methods to assign particular sigma factors to individual promoters of different classes. In vitro transcription assays and measurements of promoter activity using the overexpression of a single sigma factor gene and the transcriptional fusion of the promoter to the gfpuv reporter gene enabled us to reliably define the sigma factor dependency of promoters. To document the strengths of these methods, we tested examples of respective promoters for each C. glutamicum sigma factor. Promoters of the rshA (anti-sigma for sH) and trxB1 (thioredoxin) genes were found to be sigma(H)-dependent, whereas the promoter of the sigB gene (sigma factor sigma(B)) was sigma(E)-and sigma(H)-dependent. It was confirmed that the promoter of the cg2556 gene (iron-regulated membrane protein) is sigma(C)-dependent as suggested recently by other authors. The promoter of cmt1 (trehalose corynemycolyl transferase) was found to be clearly sigma(D)-dependent. No sigma(M)-dependent promoter was identified. The typical housekeeping promoter P2sigA (sigma factor sigma(A)) was proven to be sigma(A)-dependent but also recognized by sigma(B). Similarly, the promoter of fba (fructose-1,6-bisphosphate aldolase) was confirmed to be sigma(B)-dependent but also functional with sigma(A). The study provided demonstrations of the broad applicability of the developed methods and produced original data on the analyzed promoters.

  • Název v anglickém jazyce

    Assignment of sigma factors of RNA polymerase to promoters in Corynebacterium glutamicum

  • Popis výsledku anglicky

    Corynebacterium glutamicum is an important industrial producer of various amino acids and other metabolites. The C. glutamicum genome encodes seven sigma subunits (factors) of RNA polymerase: the primary sigma factor SigA (sA), the primary-like sigma(B) and five alternative sigma factors (sigma(C), sigma(D), sigma(E), sigma(H) and sigma(M)). We have developed in vitro and in vivo methods to assign particular sigma factors to individual promoters of different classes. In vitro transcription assays and measurements of promoter activity using the overexpression of a single sigma factor gene and the transcriptional fusion of the promoter to the gfpuv reporter gene enabled us to reliably define the sigma factor dependency of promoters. To document the strengths of these methods, we tested examples of respective promoters for each C. glutamicum sigma factor. Promoters of the rshA (anti-sigma for sH) and trxB1 (thioredoxin) genes were found to be sigma(H)-dependent, whereas the promoter of the sigB gene (sigma factor sigma(B)) was sigma(E)-and sigma(H)-dependent. It was confirmed that the promoter of the cg2556 gene (iron-regulated membrane protein) is sigma(C)-dependent as suggested recently by other authors. The promoter of cmt1 (trehalose corynemycolyl transferase) was found to be clearly sigma(D)-dependent. No sigma(M)-dependent promoter was identified. The typical housekeeping promoter P2sigA (sigma factor sigma(A)) was proven to be sigma(A)-dependent but also recognized by sigma(B). Similarly, the promoter of fba (fructose-1,6-bisphosphate aldolase) was confirmed to be sigma(B)-dependent but also functional with sigma(A). The study provided demonstrations of the broad applicability of the developed methods and produced original data on the analyzed promoters.

Klasifikace

  • Druh

    J<sub>imp</sub> - Článek v periodiku v databázi Web of Science

  • CEP obor

  • OECD FORD obor

    10606 - Microbiology

Návaznosti výsledku

  • Projekt

    <a href="/cs/project/GA17-06991S" target="_blank" >GA17-06991S: Konstrukce modelu regulační sítě řízené sigma faktory RNA polymerasy v Corynebacterium glutamicum</a><br>

  • Návaznosti

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Ostatní

  • Rok uplatnění

    2017

  • Kód důvěrnosti údajů

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Údaje specifické pro druh výsledku

  • Název periodika

    AMB Express

  • ISSN

    2191-0855

  • e-ISSN

  • Svazek periodika

    7

  • Číslo periodika v rámci svazku

    JUN 23

  • Stát vydavatele periodika

    DE - Spolková republika Německo

  • Počet stran výsledku

    13

  • Strana od-do

  • Kód UT WoS článku

    000404051700002

  • EID výsledku v databázi Scopus

    2-s2.0-85021293503