Overlap of Promoter Recognition Specificity of Stress Response Sigma Factors SigD and SigH in Corynebacterium glutamicum ATCC 13032

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F19%3A00503922" target="_blank" >RIV/61388971:_____/19:00503922 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11320/19:10406015

Výsledek na webu

<a href="https://www.frontiersin.org/articles/10.3389/fmicb.2018.03287/full" target="_blank" >https://www.frontiersin.org/articles/10.3389/fmicb.2018.03287/full</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fmicb.2018.03287" target="_blank" >10.3389/fmicb.2018.03287</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Overlap of Promoter Recognition Specificity of Stress Response Sigma Factors SigD and SigH in Corynebacterium glutamicum ATCC 13032

Popis výsledku v původním jazyce

Corynebacterium glutamicum ATCC 13032 harbors five sigma subunits of RNA polymerase belonging to Group IV, also called extracytoplasmic function (ECF) sigma factors. These factors sigma(C), sigma(D), sigma(E), sigma(H), and sigma(M) are mostly involved in stress responses. The role of sigma(D) consists in the control of cell wall integrity. The sigma(D) regulon is involved in the synthesis of components of the mycomembrane which is part of the cell wall in C. glutamicum. RNA sequencing of the transcriptome from a strain overexpressing the sigD gene provided 29 potential sigma(D)-controlled genes and enabled us to precisely localize their transcriptional start sites. Analysis of the respective promoters by both in vitro transcription and the in vivo two-plasmid assay confirmed that transcription of 11 of the tested genes is directly sigma(D)-dependent. The key sequence elements of all promoters were found to be identical or closely similar to the motifs -35 GTAAC(A)/G and -10 GAT. Nearly all of these sigma(D)-dependent promoters were also active to a much lower extent with sigma(H) in vivo and one (Pcg0607) also in vitro, although the known highly conserved consensus sequence of the sigma(H)-dependent promoters is different (-35 GGAA(T)/C and -10 GTT). In addition to the activity of sigma(H) at the sigma(D)-controlled promoters, we discovered separated or overlapping sigma(A) - or sigma(B)-regulated or sigma(H)-regulated promoters within the upstream region of 8 genes of the sigma(D)-regulon. phenol in the cultivation medium acts as a stress factor inducing expression of some sigma(D)-dependent genes. Computer modeling revealed that sigma(H) binds to the promoter DNA in a similar manner as sigma(D) to the analogous promoter elements. The homology models together with mutational analysis showed that the key amino acids, Ala 60 in sigma(D) and Lys 53 in sigma(H), bind to the second nucleotide within the respective -10 promoter elements (GAT and GTT, respectively).

Název v anglickém jazyce

Overlap of Promoter Recognition Specificity of Stress Response Sigma Factors SigD and SigH in Corynebacterium glutamicum ATCC 13032

Popis výsledku anglicky

Corynebacterium glutamicum ATCC 13032 harbors five sigma subunits of RNA polymerase belonging to Group IV, also called extracytoplasmic function (ECF) sigma factors. These factors sigma(C), sigma(D), sigma(E), sigma(H), and sigma(M) are mostly involved in stress responses. The role of sigma(D) consists in the control of cell wall integrity. The sigma(D) regulon is involved in the synthesis of components of the mycomembrane which is part of the cell wall in C. glutamicum. RNA sequencing of the transcriptome from a strain overexpressing the sigD gene provided 29 potential sigma(D)-controlled genes and enabled us to precisely localize their transcriptional start sites. Analysis of the respective promoters by both in vitro transcription and the in vivo two-plasmid assay confirmed that transcription of 11 of the tested genes is directly sigma(D)-dependent. The key sequence elements of all promoters were found to be identical or closely similar to the motifs -35 GTAAC(A)/G and -10 GAT. Nearly all of these sigma(D)-dependent promoters were also active to a much lower extent with sigma(H) in vivo and one (Pcg0607) also in vitro, although the known highly conserved consensus sequence of the sigma(H)-dependent promoters is different (-35 GGAA(T)/C and -10 GTT). In addition to the activity of sigma(H) at the sigma(D)-controlled promoters, we discovered separated or overlapping sigma(A) - or sigma(B)-regulated or sigma(H)-regulated promoters within the upstream region of 8 genes of the sigma(D)-regulon. phenol in the cultivation medium acts as a stress factor inducing expression of some sigma(D)-dependent genes. Computer modeling revealed that sigma(H) binds to the promoter DNA in a similar manner as sigma(D) to the analogous promoter elements. The homology models together with mutational analysis showed that the key amino acids, Ala 60 in sigma(D) and Lys 53 in sigma(H), bind to the second nucleotide within the respective -10 promoter elements (GAT and GTT, respectively).

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/GA17-06991S" target="_blank" >GA17-06991S: Konstrukce modelu regulační sítě řízené sigma faktory RNA polymerasy v Corynebacterium glutamicum</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Frontiers in Microbiology

ISSN

1664-302X

e-ISSN

—

Svazek periodika

9

Číslo periodika v rámci svazku

JAN 9

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

17

Strana od-do

3287

Kód UT WoS článku

000455273200001

EID výsledku v databázi Scopus

2-s2.0-85062624877