Raman and fluorescence microscopy sensing energy-transducing and energy-storing structures in microalgae

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F16%3A00463694" target="_blank" >RIV/61388971:_____/16:00463694 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11320/16:10332428

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.algal.2016.03.016" target="_blank" >http://dx.doi.org/10.1016/j.algal.2016.03.016</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.algal.2016.03.016" target="_blank" >10.1016/j.algal.2016.03.016</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Raman and fluorescence microscopy sensing energy-transducing and energy-storing structures in microalgae

Popis výsledku v původním jazyce

Chlorophylls and carotenoids are core components of photosynthetic energy transduction in algal cells, whereas starch, lipids, and polyphosphates represent energy reserves. All these biomolecules exhibit characteristic molecular vibrations that were sensed and localized in individual cells of Desmodesmus quadricauda and Chlorella vulgaris by confocal Raman microscopy. In the same cells, fluorescence of chlorophylls and of lipid bodies stained with Nile Red was mapped using the same instrument. In the first of the three consecutive scans, a low-power and short-exposure excitation by a green 532 nm laser was used to map chlorophyll fluorescence. In the second scan, the full power of a 785 nm laser was used for Raman mapping of carotenoids.

Název v anglickém jazyce

Raman and fluorescence microscopy sensing energy-transducing and energy-storing structures in microalgae

Popis výsledku anglicky

Chlorophylls and carotenoids are core components of photosynthetic energy transduction in algal cells, whereas starch, lipids, and polyphosphates represent energy reserves. All these biomolecules exhibit characteristic molecular vibrations that were sensed and localized in individual cells of Desmodesmus quadricauda and Chlorella vulgaris by confocal Raman microscopy. In the same cells, fluorescence of chlorophylls and of lipid bodies stained with Nile Red was mapped using the same instrument. In the first of the three consecutive scans, a low-power and short-exposure excitation by a green 532 nm laser was used to map chlorophyll fluorescence. In the second scan, the full power of a 785 nm laser was used for Raman mapping of carotenoids.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

—

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Algal Research-Biomass Biofuels and Bioproducts

ISSN

2211-9264

e-ISSN

—

Svazek periodika

16

Číslo periodika v rámci svazku

June

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

224-232

Kód UT WoS článku

000375610000026

EID výsledku v databázi Scopus

2-s2.0-84961129437