Antibody repertoire development in fetal and neonatal piglets. XXIV. Hypothesis: The ileal Peyer patches (IPP) are the major source of primary, undiversified IgA antibodies in newborn piglets
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F16%3A00467813" target="_blank" >RIV/61388971:_____/16:00467813 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.1016/j.dci.2016.07.020" target="_blank" >http://dx.doi.org/10.1016/j.dci.2016.07.020</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.dci.2016.07.020" target="_blank" >10.1016/j.dci.2016.07.020</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Antibody repertoire development in fetal and neonatal piglets. XXIV. Hypothesis: The ileal Peyer patches (IPP) are the major source of primary, undiversified IgA antibodies in newborn piglets
Popis výsledku v původním jazyce
The Heal Peyers patches (IPP) of newborn germfree (GF) piglets were isolated into blind loops and the piglets colonized with a defined probiotic microflora. After 5 weeks, IgA levels in the intestinal lavage (IL) of loop piglets remained at GF levels and IgM comprised similar to 70% while in controls, IgA levels were elevated 5-fold and comprised similar to 70% of total Igs. Loop piglets also had. reduced serum IgA levels suggesting the source of serum IgA had been interrupted. The isotype profile for loop contents was intermediate between that in the IL of GF and probiotic controls. Surprisingly, colonization alone did not result in repertoire diversification in the IPP. Rather, colonization promoted pronounced proliferation of fully switched IgA(+)IgM(-) B cells in the IPP that supply early, non-diversified "natural" SIgA antibodies to the gut lumen and a primary IgA response in serum.
Název v anglickém jazyce
Antibody repertoire development in fetal and neonatal piglets. XXIV. Hypothesis: The ileal Peyer patches (IPP) are the major source of primary, undiversified IgA antibodies in newborn piglets
Popis výsledku anglicky
The Heal Peyers patches (IPP) of newborn germfree (GF) piglets were isolated into blind loops and the piglets colonized with a defined probiotic microflora. After 5 weeks, IgA levels in the intestinal lavage (IL) of loop piglets remained at GF levels and IgM comprised similar to 70% while in controls, IgA levels were elevated 5-fold and comprised similar to 70% of total Igs. Loop piglets also had. reduced serum IgA levels suggesting the source of serum IgA had been interrupted. The isotype profile for loop contents was intermediate between that in the IL of GF and probiotic controls. Surprisingly, colonization alone did not result in repertoire diversification in the IPP. Rather, colonization promoted pronounced proliferation of fully switched IgA(+)IgM(-) B cells in the IPP that supply early, non-diversified "natural" SIgA antibodies to the gut lumen and a primary IgA response in serum.
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
EC - Imunologie
OECD FORD obor
—
Návaznosti výsledku
Projekt
<a href="/cs/project/GA15-02274S" target="_blank" >GA15-02274S: Úloha lehkých řetězců protilátek ve vývoji prasečích B buněk</a><br>
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2016
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Developmental and Comparative Immunology
ISSN
0145-305X
e-ISSN
—
Svazek periodika
65
Číslo periodika v rámci svazku
December
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
12
Strana od-do
340-351
Kód UT WoS článku
000384388400038
EID výsledku v databázi Scopus
2-s2.0-84982243068