Diversity and identification of Neofabraea species causing bull’s eye rot in the Czech Republic

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F17%3A00467182" target="_blank" >RIV/61388971:_____/17:00467182 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/17:10368575 RIV/00027006:_____/17:00003968

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s10658-016-1036-1" target="_blank" >http://dx.doi.org/10.1007/s10658-016-1036-1</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10658-016-1036-1" target="_blank" >10.1007/s10658-016-1036-1</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Diversity and identification of Neofabraea species causing bull’s eye rot in the Czech Republic

Popis výsledku v původním jazyce

Four Neofabraea species are responsible for bull´s eye rot, which is an important postharvest disease of apples and pears. The species diversity of its causal agents in Europe has not been thoroughly explored using molecular genetic methods. Eighty-one Neofabraea isolates were obtained mostly from apples with bull´s eye rot symptoms in the Czech Republic over a two year period. The isolates were identified using PCR fingerprinting and DNA sequencing of the ITS rDNA region, the mitochondrial SSU rDNA and the Beta-tubulin and EF1alfa genes. The most common species was N. alba (89 percent), followed by N. perennans (5 percent) and N. kienholzii (5 percent). This is the third published record of N. kienholzii in Europe. The species identity of the isolate CPPF507, which was placed close to N. kienholzii, remains unclear. EF1alfa was shown to be a suitable marker for the identification of species of the genus Neofabraea and was comparable to the previously used Beta-tubulin gene. Furthermore, the aggressiveness of individual species was compared and species distribution across Europe was summarized. N. perennans and isolate CPPF507 proved to be the most aggressive, whereas the least aggressive was N. kienholzii. Two N. alba isolates isolated from symptomless apple fruits and leaves were pathogenic to apples in the infection tests.

Název v anglickém jazyce

Diversity and identification of Neofabraea species causing bull’s eye rot in the Czech Republic

Popis výsledku anglicky

Four Neofabraea species are responsible for bull´s eye rot, which is an important postharvest disease of apples and pears. The species diversity of its causal agents in Europe has not been thoroughly explored using molecular genetic methods. Eighty-one Neofabraea isolates were obtained mostly from apples with bull´s eye rot symptoms in the Czech Republic over a two year period. The isolates were identified using PCR fingerprinting and DNA sequencing of the ITS rDNA region, the mitochondrial SSU rDNA and the Beta-tubulin and EF1alfa genes. The most common species was N. alba (89 percent), followed by N. perennans (5 percent) and N. kienholzii (5 percent). This is the third published record of N. kienholzii in Europe. The species identity of the isolate CPPF507, which was placed close to N. kienholzii, remains unclear. EF1alfa was shown to be a suitable marker for the identification of species of the genus Neofabraea and was comparable to the previously used Beta-tubulin gene. Furthermore, the aggressiveness of individual species was compared and species distribution across Europe was summarized. N. perennans and isolate CPPF507 proved to be the most aggressive, whereas the least aggressive was N. kienholzii. Two N. alba isolates isolated from symptomless apple fruits and leaves were pathogenic to apples in the infection tests.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

European journal of plant pathology

ISSN

0929-1873

e-ISSN

—

Svazek periodika

147

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

11

Strana od-do

—

Kód UT WoS článku

000394148400017

EID výsledku v databázi Scopus

2-s2.0-84983402030