Impact of inhibitors of amino acid, protein, and RNA synthesis on C allocation in the diatom Chaetoceros muellerii: a FTIR approach

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F17%3A00476470" target="_blank" >RIV/61388971:_____/17:00476470 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.4490/algae.2017.32.6.6" target="_blank" >http://dx.doi.org/10.4490/algae.2017.32.6.6</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.4490/algae.2017.32.6.6" target="_blank" >10.4490/algae.2017.32.6.6</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Impact of inhibitors of amino acid, protein, and RNA synthesis on C allocation in the diatom Chaetoceros muellerii: a FTIR approach

Popis výsledku v původním jazyce

Fourier Transform Infrared (FTIR) spectroscopy was used to study carbon allocation patterns in response to N-starvation in the nearly ubiquitous diatom Chaetoceros muellerii. The role of gene expression, protein synthesis and transamination on the organic composition of cells was tested by using specific inhibitors. The results show that inhibition of key processes in algal metabolism influence the macromolecular composition of cells and and prior cell nutritional state can influence a cell's response to changing nutrient availability. The allocation of C can thus lead to different organic composition depending on the nutritional context, with obvious repercussions for the trophic web. This also shows that C allocation in algal cells is highly flexible and that C (and the energy associated with its allocation) can be variably and rapidly partitioned in algal cells in response to relatively short term perturbations. Furthermore, the data confirm and extend the utility of infrared spectroscopy as a probe of the metabolic state of autotrophic cells.

Název v anglickém jazyce

Impact of inhibitors of amino acid, protein, and RNA synthesis on C allocation in the diatom Chaetoceros muellerii: a FTIR approach

Popis výsledku anglicky

Fourier Transform Infrared (FTIR) spectroscopy was used to study carbon allocation patterns in response to N-starvation in the nearly ubiquitous diatom Chaetoceros muellerii. The role of gene expression, protein synthesis and transamination on the organic composition of cells was tested by using specific inhibitors. The results show that inhibition of key processes in algal metabolism influence the macromolecular composition of cells and and prior cell nutritional state can influence a cell's response to changing nutrient availability. The allocation of C can thus lead to different organic composition depending on the nutritional context, with obvious repercussions for the trophic web. This also shows that C allocation in algal cells is highly flexible and that C (and the energy associated with its allocation) can be variably and rapidly partitioned in algal cells in response to relatively short term perturbations. Furthermore, the data confirm and extend the utility of infrared spectroscopy as a probe of the metabolic state of autotrophic cells.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Algae

ISSN

1226-2617

e-ISSN

—

Svazek periodika

32

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

KR - Korejská republika

Počet stran výsledku

10

Strana od-do

161-170

Kód UT WoS článku

000404833700006

EID výsledku v databázi Scopus

2-s2.0-85021128440