Teicoplanin resistance in Staphylococcus haemolyticus is associated with mutations in histidine kinases VraS and WalK

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F18%3A00488776" target="_blank" >RIV/61388971:_____/18:00488776 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.diagmicrobio.2017.11.007" target="_blank" >http://dx.doi.org/10.1016/j.diagmicrobio.2017.11.007</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.diagmicrobio.2017.11.007" target="_blank" >10.1016/j.diagmicrobio.2017.11.007</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Teicoplanin resistance in Staphylococcus haemolyticus is associated with mutations in histidine kinases VraS and WalK

Popis výsledku v původním jazyce

We investigated the genetic basis of glycopeptide resistance in laboratory-derived strains of S. haemolyticus with emphasis on differences between vancomycin and teicoplanin. The genomes of two stable teicoplanin-resistant laboratory mutants selected on vancomycin or teicoplanin were sequenced and compared to parental S. haemolyticus strain W2/124. Only the two non-synonymous mutations, VraS Q289K and Walk V550 L were identified. No other mutations or genome rearrangements were detected. Increased cell wall thickness, resistance to lysostaphin-induced lysis and adaptation of cell growth rates specifically to teicoplanin were phenotypes observed in a sequenced strain with the VraS Q289K mutation. Neither of the VraS Q289K and Wall< V550 L mutations was present in the genomes of 121 S. haemolyticus clinical isolates. However, all but two of the teicoplanin resistant strains carried non-synonymous SNP5 in vraSRTU and walKR-YycHIJ operons pointing to their importance for the glycopeptide resistance. (C) 2017 Elsevier Inc. All rights reserved.

Název v anglickém jazyce

Teicoplanin resistance in Staphylococcus haemolyticus is associated with mutations in histidine kinases VraS and WalK

Popis výsledku anglicky

We investigated the genetic basis of glycopeptide resistance in laboratory-derived strains of S. haemolyticus with emphasis on differences between vancomycin and teicoplanin. The genomes of two stable teicoplanin-resistant laboratory mutants selected on vancomycin or teicoplanin were sequenced and compared to parental S. haemolyticus strain W2/124. Only the two non-synonymous mutations, VraS Q289K and Walk V550 L were identified. No other mutations or genome rearrangements were detected. Increased cell wall thickness, resistance to lysostaphin-induced lysis and adaptation of cell growth rates specifically to teicoplanin were phenotypes observed in a sequenced strain with the VraS Q289K mutation. Neither of the VraS Q289K and Wall< V550 L mutations was present in the genomes of 121 S. haemolyticus clinical isolates. However, all but two of the teicoplanin resistant strains carried non-synonymous SNP5 in vraSRTU and walKR-YycHIJ operons pointing to their importance for the glycopeptide resistance. (C) 2017 Elsevier Inc. All rights reserved.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Diagnostic Microbiology and Infectious Disease

ISSN

0732-8893

e-ISSN

—

Svazek periodika

90

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

233-240

Kód UT WoS článku

000426228700015

EID výsledku v databázi Scopus

2-s2.0-85037725101