Molecular epidemiology of Trichophyton quinckeanum - a zoophilic dermatophyte on the rise

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F18%3A00488958" target="_blank" >RIV/61388971:_____/18:00488958 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1111/ddg.13408" target="_blank" >http://dx.doi.org/10.1111/ddg.13408</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/ddg.13408" target="_blank" >10.1111/ddg.13408</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Molecular epidemiology of Trichophyton quinckeanum - a zoophilic dermatophyte on the rise

Popis výsledku v původním jazyce

Background: Formerly only referred to as a subspecies (T. mentagrophytes var. quinckeanum), T. quinckeanum once again constitutes a distinct species according to the updated taxonomy of dermatophytes. nPatients and methods: During routine diagnostic tests conducted at the Mycology Laboratory, Molbis, Germany, between 11/2013 to 1/2017 (three years and three months), all specimens sent in were examined for T. quinckeanum. Molecular biology methods employed included: 1) DNA hybridization (PCR ELISA), 2) gene sequencing of the ITS region and TEF-1 alpha, and 3) in some cases, MALDI-TOF mass spectrometry. nResults: Overall, 62 strains of T. quinckeanum were found. Sixty-eight percent of patients were female, 43% were children and adolescents (<= 19 years of age). Cats were a frequent source of infection. Sequencing of all 62 strains revealed a concordance of 100 % with T. quinckeanum sequences contained in the NCBI database. Isolates analyzed by MALDI-TOF mass spectrometry showed specific spectra. nConclusions: In Germany, the zoophilic dermatophyte T. quinckeanum currently appears to be more prevalent than expected. T. quinckeanum strains were isolated from children and adults with dermatomycosis and tinea capitis. Sources of infection with T. quinckeanum include small rodents (mice), horses, and - remarkably commonly - cats. Given that unequivocal morphological identification of this dermatophyte is not always possible, molecular methods have to be employed in the diagnosis.

Název v anglickém jazyce

Molecular epidemiology of Trichophyton quinckeanum - a zoophilic dermatophyte on the rise

Popis výsledku anglicky

Background: Formerly only referred to as a subspecies (T. mentagrophytes var. quinckeanum), T. quinckeanum once again constitutes a distinct species according to the updated taxonomy of dermatophytes. nPatients and methods: During routine diagnostic tests conducted at the Mycology Laboratory, Molbis, Germany, between 11/2013 to 1/2017 (three years and three months), all specimens sent in were examined for T. quinckeanum. Molecular biology methods employed included: 1) DNA hybridization (PCR ELISA), 2) gene sequencing of the ITS region and TEF-1 alpha, and 3) in some cases, MALDI-TOF mass spectrometry. nResults: Overall, 62 strains of T. quinckeanum were found. Sixty-eight percent of patients were female, 43% were children and adolescents (<= 19 years of age). Cats were a frequent source of infection. Sequencing of all 62 strains revealed a concordance of 100 % with T. quinckeanum sequences contained in the NCBI database. Isolates analyzed by MALDI-TOF mass spectrometry showed specific spectra. nConclusions: In Germany, the zoophilic dermatophyte T. quinckeanum currently appears to be more prevalent than expected. T. quinckeanum strains were isolated from children and adults with dermatomycosis and tinea capitis. Sources of infection with T. quinckeanum include small rodents (mice), horses, and - remarkably commonly - cats. Given that unequivocal morphological identification of this dermatophyte is not always possible, molecular methods have to be employed in the diagnosis.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/NV17-31269A" target="_blank" >NV17-31269A: Molekulární epidemiologie a zdokonalení identifikace dermatofyt v České republice</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

JDDG

ISSN

1610-0379

e-ISSN

—

Svazek periodika

16

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

12

Strana od-do

21-32

Kód UT WoS článku

000419640700004

EID výsledku v databázi Scopus

2-s2.0-85040232653