Potential of the strain Raoultella sp KDF8 for removal of analgesics

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F18%3A00489382" target="_blank" >RIV/61388971:_____/18:00489382 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/86652036:_____/18:00489382 RIV/00216208:11310/18:10368407

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s12223-017-0563-2" target="_blank" >http://dx.doi.org/10.1007/s12223-017-0563-2</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s12223-017-0563-2" target="_blank" >10.1007/s12223-017-0563-2</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Potential of the strain Raoultella sp KDF8 for removal of analgesics

Popis výsledku v původním jazyce

The bacterial strain KDF8 capable of growth in the presence of diclofenac and codeine analgesics was obtained after chemical mutagenesis of nature isolates from polluted soils. The strain KDF8 was identified as Raoultella sp. based on its morphology, biochemical properties, and 16S rRNA gene sequence. It was deposited in the Czech Collection of Microorganisms under the number CCM 8678. A growing culture efficiently removed diclofenac (92% removal) and partially also codeine (about 30% degradation) from culture supernatants within 72 h at 28 degrees C. The degradation of six analgesics by the whole cell catalyst was investigated in detail. The maximum degradation of diclofenac (91%) by the catalyst was achieved at pH(INI) of 7 (1 g/L diclofenac). The specific removal rate at high concentrations of diclofenac and codeine increased up to 16.5 mg/g(CDW) per h and 5.1 mg/g(CDW) per h, respectively. HPLC analysis identified 4'-hydroxydiclofenac as a major metabolite of diclofenac transformation and 14-hydroxycodeinone as codeine transformation product. The analgesics ibuprofen and ketoprofen were also removed, albeit to a lower extent of 3.2 and 2.0 mg/g(CDW) per h, respectively. Naproxen and mefenamic acid were not degraded.

Název v anglickém jazyce

Potential of the strain Raoultella sp KDF8 for removal of analgesics

Popis výsledku anglicky

The bacterial strain KDF8 capable of growth in the presence of diclofenac and codeine analgesics was obtained after chemical mutagenesis of nature isolates from polluted soils. The strain KDF8 was identified as Raoultella sp. based on its morphology, biochemical properties, and 16S rRNA gene sequence. It was deposited in the Czech Collection of Microorganisms under the number CCM 8678. A growing culture efficiently removed diclofenac (92% removal) and partially also codeine (about 30% degradation) from culture supernatants within 72 h at 28 degrees C. The degradation of six analgesics by the whole cell catalyst was investigated in detail. The maximum degradation of diclofenac (91%) by the catalyst was achieved at pH(INI) of 7 (1 g/L diclofenac). The specific removal rate at high concentrations of diclofenac and codeine increased up to 16.5 mg/g(CDW) per h and 5.1 mg/g(CDW) per h, respectively. HPLC analysis identified 4'-hydroxydiclofenac as a major metabolite of diclofenac transformation and 14-hydroxycodeinone as codeine transformation product. The analgesics ibuprofen and ketoprofen were also removed, albeit to a lower extent of 3.2 and 2.0 mg/g(CDW) per h, respectively. Naproxen and mefenamic acid were not degraded.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/TH02030337" target="_blank" >TH02030337: Stanovení distribuce farmakologicky aktivních látek a jejich biologická degradace v rámci procesů čištění odpadních vod</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Folia Microbiologica

ISSN

0015-5632

e-ISSN

—

Svazek periodika

63

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

10

Strana od-do

273-282

Kód UT WoS článku

000430317100002

EID výsledku v databázi Scopus

2-s2.0-85033434424