Characterization of the catabolic pathway of diclofenac in Raoultella sp. KDF8

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F19%3A00504027" target="_blank" >RIV/61388971:_____/19:00504027 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0964830518310631?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0964830518310631?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ibiod.2018.11.013" target="_blank" >10.1016/j.ibiod.2018.11.013</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Characterization of the catabolic pathway of diclofenac in Raoultella sp. KDF8

Popis výsledku v původním jazyce

The removal of diclofenac, which is a widely used non-steroidal anti-inflammatory drug and a dangerous environmental contaminant of surface water, was studied. Its complete degradation by Raoultella sp. KDF8 to primary metabolic products was described with individual metabolites identified by mass spectrometry. The Raoultella strain KDF8 was cultivated with unlabelled and (13)C6-labelled diclofenac. A total of 32 compounds were detected as intermediates in diclofenac metabolism by high resolution tandem mass spectrometry. Furthermore, proteomic analysis showed that the presence of diclofenac resulted in the upregulation of 24 enzymes associated with the benzoate, catechol, protocatechuate and ketoadipate pathways. The presence of genes encoding specific enzymes, such as catechol 1,2-dioxygenase, protocatechuate 3,4-dioxygenase and quercetin 2,3-dioxygenase, has been verified by sequence analysis of PCR products. The description of diclofenac metabolism by strain KDF8 provides a detailed view of the degradation of xenobiotic aromatic compounds by microorganisms in general.

Název v anglickém jazyce

Characterization of the catabolic pathway of diclofenac in Raoultella sp. KDF8

Popis výsledku anglicky

The removal of diclofenac, which is a widely used non-steroidal anti-inflammatory drug and a dangerous environmental contaminant of surface water, was studied. Its complete degradation by Raoultella sp. KDF8 to primary metabolic products was described with individual metabolites identified by mass spectrometry. The Raoultella strain KDF8 was cultivated with unlabelled and (13)C6-labelled diclofenac. A total of 32 compounds were detected as intermediates in diclofenac metabolism by high resolution tandem mass spectrometry. Furthermore, proteomic analysis showed that the presence of diclofenac resulted in the upregulation of 24 enzymes associated with the benzoate, catechol, protocatechuate and ketoadipate pathways. The presence of genes encoding specific enzymes, such as catechol 1,2-dioxygenase, protocatechuate 3,4-dioxygenase and quercetin 2,3-dioxygenase, has been verified by sequence analysis of PCR products. The description of diclofenac metabolism by strain KDF8 provides a detailed view of the degradation of xenobiotic aromatic compounds by microorganisms in general.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Biodeterioration & Biodegradation

ISSN

0964-8305

e-ISSN

—

Svazek periodika

137

Číslo periodika v rámci svazku

FEB2019

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

7

Strana od-do

88-94

Kód UT WoS článku

000458470800010

EID výsledku v databázi Scopus

2-s2.0-85057591494