sigma(I) from Bacillus subtilis: Impact on Gene Expression and Characterization of sigma(I)-Dependent Transcription That Requires New Types of Promoters with Extended-35 and-10 Elements

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F18%3A00493283" target="_blank" >RIV/61388971:_____/18:00493283 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/18:10383396

Výsledek na webu

<a href="http://dx.doi.org/10.1128/JB.00251-18" target="_blank" >http://dx.doi.org/10.1128/JB.00251-18</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1128/JB.00251-18" target="_blank" >10.1128/JB.00251-18</a>

Jazyk výsledku

angličtina

Název v původním jazyce

sigma(I) from Bacillus subtilis: Impact on Gene Expression and Characterization of sigma(I)-Dependent Transcription That Requires New Types of Promoters with Extended-35 and-10 Elements

Popis výsledku v původním jazyce

The sigma(I) sigma factor from Bacillus subtilis is a a factor associated with RNA polymerase (RNAP) that was previously implicated in adaptation of the cell to elevated temperature. Here, we provide a comprehensive characterization of this transcriptional regulator. By transcriptome sequencing (RNA-seq) of wild-type (wt) and sigma(I)-null strains at 37 degrees C and 52 degrees C, we identified -130 genes affected by the absence of sigma(I). Further analysis revealed that the majority of these genes were affected indirectly by sigma(I). The sigma(I) regulon, i.e., the genes directly regulated by sigma(I), consists of 16 genes, of which eight (the dhb and yku operons) are involved in iron metabolism. The involvement of sigma(I) in iron metabolism was confirmed phenotypically. Next, we set up an in vitro transcription system and defined and experimentally validated the promoter sequence logo that, in addition to -35 and -10 regions, also contains extended -35 and -10 motifs. Thus, sigma(I)-dependent promoters are relatively information rich in comparison with most other promoters. In summary, this study supplies information about the least-explored sigma factor from the industrially important model organism B. subtilis. n

Název v anglickém jazyce

sigma(I) from Bacillus subtilis: Impact on Gene Expression and Characterization of sigma(I)-Dependent Transcription That Requires New Types of Promoters with Extended-35 and-10 Elements

Popis výsledku anglicky

The sigma(I) sigma factor from Bacillus subtilis is a a factor associated with RNA polymerase (RNAP) that was previously implicated in adaptation of the cell to elevated temperature. Here, we provide a comprehensive characterization of this transcriptional regulator. By transcriptome sequencing (RNA-seq) of wild-type (wt) and sigma(I)-null strains at 37 degrees C and 52 degrees C, we identified -130 genes affected by the absence of sigma(I). Further analysis revealed that the majority of these genes were affected indirectly by sigma(I). The sigma(I) regulon, i.e., the genes directly regulated by sigma(I), consists of 16 genes, of which eight (the dhb and yku operons) are involved in iron metabolism. The involvement of sigma(I) in iron metabolism was confirmed phenotypically. Next, we set up an in vitro transcription system and defined and experimentally validated the promoter sequence logo that, in addition to -35 and -10 regions, also contains extended -35 and -10 motifs. Thus, sigma(I)-dependent promoters are relatively information rich in comparison with most other promoters. In summary, this study supplies information about the least-explored sigma factor from the industrially important model organism B. subtilis. n

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Bacteriology

ISSN

0021-9193

e-ISSN

—

Svazek periodika

200

Číslo periodika v rámci svazku

17

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

15

Strana od-do

—

Kód UT WoS článku

000441290900010

EID výsledku v databázi Scopus

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