Deterioration of irradiation/high-temperature pretreated, linear low-density polyethylene (LLDPE) by Bacillus amyloliquefaciens

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F18%3A00496242" target="_blank" >RIV/61388971:_____/18:00496242 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.ibiod.2018.04.014" target="_blank" >http://dx.doi.org/10.1016/j.ibiod.2018.04.014</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ibiod.2018.04.014" target="_blank" >10.1016/j.ibiod.2018.04.014</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Deterioration of irradiation/high-temperature pretreated, linear low-density polyethylene (LLDPE) by Bacillus amyloliquefaciens

Popis výsledku v původním jazyce

A Bacillus amyloliquefaciens strain was isolated from composted plastics and identified using microbial identification system BIOLOG and 16S rDNA sequences. The capability to attack virgin and gamma-irradiation/high temperature-pretreated LLDPE films was investigated for comparison to evaluate the effect of pretreatment. A biodeteriorating effect characterized by low weight reductions of 1,1 +/- 0.3 to 3.2 +/- 1.3% was observed with the pretreated LLDPE within 40-60 days. The precision of the gravimetric method was sometimes negatively affected by the fragmentation of plastic films during the biological treatment or by traces of microbial biofilm firmly adhering to the plastic material. FTIR spectra before and after 60-day treatment indicated a decrease of carbonyl band and flattening of the 1300-1100 cm(-1) zone due to bacterial action. GPC showed an increase of M-n and M-w of 2300-3700 and 32 200-35 500 g mol(-1), respectively, and a decrease of polydispersity index suggesting presence of low molar weight LLDPE oligomers in pretreated LLDPE. The analysis of crystallinity and melting enthalpy detected the removal of oligomers during biodeterioration. ESI-MS analysis of the medium after 60-day biotreatment of pretreated LLDPE showed a presence of 3-hydroxybutyrate oligomers linked to the disappearance of low molar weight LLDPE oligomers that was not observed with virgin LLDPE. Coincidence of the removal of LLDPE oligomers and the appearance of 3-hydroxybutyrate oligomers suggested metabolization of low molecular LLDPE fractions present in the pretreated LLDPE by B. amyloliquefaciens. The experiments with virgin LLDPE demonstrated a positive effect of the pretreatment.

Název v anglickém jazyce

Deterioration of irradiation/high-temperature pretreated, linear low-density polyethylene (LLDPE) by Bacillus amyloliquefaciens

Popis výsledku anglicky

A Bacillus amyloliquefaciens strain was isolated from composted plastics and identified using microbial identification system BIOLOG and 16S rDNA sequences. The capability to attack virgin and gamma-irradiation/high temperature-pretreated LLDPE films was investigated for comparison to evaluate the effect of pretreatment. A biodeteriorating effect characterized by low weight reductions of 1,1 +/- 0.3 to 3.2 +/- 1.3% was observed with the pretreated LLDPE within 40-60 days. The precision of the gravimetric method was sometimes negatively affected by the fragmentation of plastic films during the biological treatment or by traces of microbial biofilm firmly adhering to the plastic material. FTIR spectra before and after 60-day treatment indicated a decrease of carbonyl band and flattening of the 1300-1100 cm(-1) zone due to bacterial action. GPC showed an increase of M-n and M-w of 2300-3700 and 32 200-35 500 g mol(-1), respectively, and a decrease of polydispersity index suggesting presence of low molar weight LLDPE oligomers in pretreated LLDPE. The analysis of crystallinity and melting enthalpy detected the removal of oligomers during biodeterioration. ESI-MS analysis of the medium after 60-day biotreatment of pretreated LLDPE showed a presence of 3-hydroxybutyrate oligomers linked to the disappearance of low molar weight LLDPE oligomers that was not observed with virgin LLDPE. Coincidence of the removal of LLDPE oligomers and the appearance of 3-hydroxybutyrate oligomers suggested metabolization of low molecular LLDPE fractions present in the pretreated LLDPE by B. amyloliquefaciens. The experiments with virgin LLDPE demonstrated a positive effect of the pretreatment.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Biodeterioration & Biodegradation

ISSN

0964-8305

e-ISSN

—

Svazek periodika

132

Číslo periodika v rámci svazku

AUG 2018

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

9

Strana od-do

259-267

Kód UT WoS článku

000447358900030

EID výsledku v databázi Scopus

2-s2.0-85047350505