Ability of Trichoderma hamatum Isolated from Plastics-Polluted Environments to Attack Petroleum-Based, Synthetic Polymer Films

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61988987%3A17310%2F20%3AA21027Z7" target="_blank" >RIV/61988987:17310/20:A21027Z7 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388971:_____/20:00533324 RIV/61989100:27710/20:10245063

Výsledek na webu

<a href="http://www.mdpi.com/journal/processes" target="_blank" >http://www.mdpi.com/journal/processes</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/pr8040467" target="_blank" >10.3390/pr8040467</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Ability of Trichoderma hamatum Isolated from Plastics-Polluted Environments to Attack Petroleum-Based, Synthetic Polymer Films

Popis výsledku v původním jazyce

Microorganisms colonizing plastic waste material collected in composting-, landfill-,and anaerobic digestion plants were isolated to obtain novel strains maximally adapted to thedegradation of plastics due to long-term contact with plastic polymers. Twenty-six bacterial strainswere isolated and identified by the 16 S rRNA method, and eighteen strains of yeasts and fungi using18 S rRNA and the internal transcribed spacer ITS sequencing of the 18 S rRNA gene. In selectedstrains, the ability to degrade linear low-density polyethylene (LLDPE), low-density polyethylene(LDPE), polystyrene (PS), and polyvinyl chloride (PVC) was tested in aerobic liquid-medium cultures.An oxidative, two-step pretreatment of LLDPE and LDPE using γ- or UV-irradiation followed by ahigh-temperature treatment was carried out, and the pretreated plastics were also included in thedegradation experiments. The respective weight losses after biodegradation by Trichoderma hamatumwere: virgin and γ/T90-pretreated LLDPE (2.2 ±1.2 and 3.9 ± 0.5%), virgin and UV/T60-pretreatedLDPE (0.5 ± 0.4 and 1.3 ± 0.4%), and virgin PS (0.9 ± 0.4%). The Fourier transform infrared spectroscopy(FTIR) analysis showed that during the treatment of pretreated LLDPE, T. hamatum attacked lowmolecular weight LLDPE oligomers, reducing the functional groups (carbonyl C = O), which wasparalleled by a slight increase of the molar mass of pretreated LLDPE and a decrease of the dispersityindex, as demonstrated by gel permeation chromatography (GPC). Thermogravimetric analysis(TGA) highlighted the formation of functional groups on LLDPE due to polymer pretreatment thatfavored fungal attack at the polymer surface. The results provide insight into microbial consortia thatspontaneously colonize the surface of plastics in various environments and their capability to attackplastic polymers.

Název v anglickém jazyce

Ability of Trichoderma hamatum Isolated from Plastics-Polluted Environments to Attack Petroleum-Based, Synthetic Polymer Films

Popis výsledku anglicky

Microorganisms colonizing plastic waste material collected in composting-, landfill-,and anaerobic digestion plants were isolated to obtain novel strains maximally adapted to thedegradation of plastics due to long-term contact with plastic polymers. Twenty-six bacterial strainswere isolated and identified by the 16 S rRNA method, and eighteen strains of yeasts and fungi using18 S rRNA and the internal transcribed spacer ITS sequencing of the 18 S rRNA gene. In selectedstrains, the ability to degrade linear low-density polyethylene (LLDPE), low-density polyethylene(LDPE), polystyrene (PS), and polyvinyl chloride (PVC) was tested in aerobic liquid-medium cultures.An oxidative, two-step pretreatment of LLDPE and LDPE using γ- or UV-irradiation followed by ahigh-temperature treatment was carried out, and the pretreated plastics were also included in thedegradation experiments. The respective weight losses after biodegradation by Trichoderma hamatumwere: virgin and γ/T90-pretreated LLDPE (2.2 ±1.2 and 3.9 ± 0.5%), virgin and UV/T60-pretreatedLDPE (0.5 ± 0.4 and 1.3 ± 0.4%), and virgin PS (0.9 ± 0.4%). The Fourier transform infrared spectroscopy(FTIR) analysis showed that during the treatment of pretreated LLDPE, T. hamatum attacked lowmolecular weight LLDPE oligomers, reducing the functional groups (carbonyl C = O), which wasparalleled by a slight increase of the molar mass of pretreated LLDPE and a decrease of the dispersityindex, as demonstrated by gel permeation chromatography (GPC). Thermogravimetric analysis(TGA) highlighted the formation of functional groups on LLDPE due to polymer pretreatment thatfavored fungal attack at the polymer surface. The results provide insight into microbial consortia thatspontaneously colonize the surface of plastics in various environments and their capability to attackplastic polymers.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

20400 - Chemical engineering

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Processes

ISSN

2227-9717

e-ISSN

2227-9717

Svazek periodika

8

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

14

Strana od-do

1-14

Kód UT WoS článku

000536891900074

EID výsledku v databázi Scopus

—