Assessment of agonistic and antagonistic properties of widely used oral care antimicrobial substances toward steroid estrogenic and androgenic receptors

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F19%3A00504291" target="_blank" >RIV/61388971:_____/19:00504291 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/19:10395523

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0045653518321039?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0045653518321039?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.chemosphere.2018.11.006" target="_blank" >10.1016/j.chemosphere.2018.11.006</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Assessment of agonistic and antagonistic properties of widely used oral care antimicrobial substances toward steroid estrogenic and androgenic receptors

Popis výsledku v původním jazyce

Personal care product consumption has increased in the last decades. A typical representative ingredient, i.e., triclosan, was identified in the scientific literature as an endocrine disruptor, and its use is restricted in several applications. Oral hygiene formulations contain various compounds, including synthetic phenol derivatives, quaternary ammonium compounds (QAC5), various amides and amines, or natural essential oils containing terpenes. The aim of this paper was to explore possible endocrine-disrupting effects of these most-used compounds. For this purpose, two different assays based on recombinant yeast (BMAEREluc/ER alpha, BMAEREIuc/AR) and human cell lines (T47D, AIZ-AR) were employed to investigate the agonistic and antagonistic properties of these compounds on human estrogen and androgen receptors. The results showed that none of the compounds were indicated as agonists of the steroid receptors. However, octenidine (00', QAC-like) and hexadecylpyridinium (HOP, QAC) were able to completely inhibit both androgenic (IC50 OCT = 0.84 mu M, IC50 HDP = 1.66 mu M) and estrogenic (IC50 OCT = 0.50 mu M, IC50 HDP = 1.64 mu M) signaling pathways in a dose-dependent manner. Additionally, chlorhexidine was found to inhibit the 17 beta-estradiol response, with a similar IC50 (2.9 mu M). In contrast, the natural terpenes thymol and menthol were found to be competitive antagonists of the receptors, however, their IC50 values were higher (by orders of magnitude). We tried to estimate the risk associated with the presence of these compounds in environmental matrices by calculating hazard quotients (HQs), and the calculated HQs were found to be close to or greater than 1 only when predicted environmental concentrations were used for surface waters.

Název v anglickém jazyce

Assessment of agonistic and antagonistic properties of widely used oral care antimicrobial substances toward steroid estrogenic and androgenic receptors

Popis výsledku anglicky

Personal care product consumption has increased in the last decades. A typical representative ingredient, i.e., triclosan, was identified in the scientific literature as an endocrine disruptor, and its use is restricted in several applications. Oral hygiene formulations contain various compounds, including synthetic phenol derivatives, quaternary ammonium compounds (QAC5), various amides and amines, or natural essential oils containing terpenes. The aim of this paper was to explore possible endocrine-disrupting effects of these most-used compounds. For this purpose, two different assays based on recombinant yeast (BMAEREluc/ER alpha, BMAEREIuc/AR) and human cell lines (T47D, AIZ-AR) were employed to investigate the agonistic and antagonistic properties of these compounds on human estrogen and androgen receptors. The results showed that none of the compounds were indicated as agonists of the steroid receptors. However, octenidine (00', QAC-like) and hexadecylpyridinium (HOP, QAC) were able to completely inhibit both androgenic (IC50 OCT = 0.84 mu M, IC50 HDP = 1.66 mu M) and estrogenic (IC50 OCT = 0.50 mu M, IC50 HDP = 1.64 mu M) signaling pathways in a dose-dependent manner. Additionally, chlorhexidine was found to inhibit the 17 beta-estradiol response, with a similar IC50 (2.9 mu M). In contrast, the natural terpenes thymol and menthol were found to be competitive antagonists of the receptors, however, their IC50 values were higher (by orders of magnitude). We tried to estimate the risk associated with the presence of these compounds in environmental matrices by calculating hazard quotients (HQs), and the calculated HQs were found to be close to or greater than 1 only when predicted environmental concentrations were used for surface waters.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30108 - Toxicology

Projekt

<a href="/cs/project/GJ17-15678Y" target="_blank" >GJ17-15678Y: Potenciál mikroorganismů biodegradovat antimikrobiální látky</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Chemosphere

ISSN

0045-6535

e-ISSN

—

Svazek periodika

217

Číslo periodika v rámci svazku

FEB 2019

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

534-541

Kód UT WoS článku

000456223500058

EID výsledku v databázi Scopus

2-s2.0-85057193460