Effects of cyclin-dependent kinase activity on the coordination of growth and the cell cycle in green algae at different temperatures

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F19%3A00508530" target="_blank" >RIV/61388971:_____/19:00508530 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12310/19:43899159

Výsledek na webu

<a href="https://academic.oup.com/jxb/article/70/3/845/5160063" target="_blank" >https://academic.oup.com/jxb/article/70/3/845/5160063</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/jxb/ery391" target="_blank" >10.1093/jxb/ery391</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Effects of cyclin-dependent kinase activity on the coordination of growth and the cell cycle in green algae at different temperatures

Popis výsledku v původním jazyce

The progression of the cell cycle in green algae dividing by multiple fission is, under otherwise unlimited conditions, affected by the growth rate, set by a combination of light intensity and temperature. In this study, we compared the cell cycle characteristics of Desmodesmus quadricauda at 20 degrees C or 30 degrees C and upon shifts between these two temperatures. The duration of the cell cycle in cells grown under continuous illumination at 20 degrees C was more than double that at 30 degrees C, suggesting that it was set directly by the growth rate. Similarly, the amounts of DNA, RNA, and bulk protein content per cell at 20 degrees C were approximately double those of cells grown at the higher temperature. For the shift experiments, cells grown at either 20 degrees C or 30 degrees C were transferred to darkness to prevent further growth, and then cultivated at the same or the other temperature. Upon transfer to the lower temperature, fewer nuclei and daughter cells were produced, and not all cells were able to finish the cell cycle by division, remaining multinuclear. Correspondingly, cells placed in the dark at the higher temperature divided faster into more daughter cells than the control cells. These differences correlated with shifts in the preceding cyclin-dependent kinase activity, suggesting that cell cycle progression was not related to growth rate or cell biomass but correlated with cyclin-dependent kinase activity.

Název v anglickém jazyce

Effects of cyclin-dependent kinase activity on the coordination of growth and the cell cycle in green algae at different temperatures

Popis výsledku anglicky

The progression of the cell cycle in green algae dividing by multiple fission is, under otherwise unlimited conditions, affected by the growth rate, set by a combination of light intensity and temperature. In this study, we compared the cell cycle characteristics of Desmodesmus quadricauda at 20 degrees C or 30 degrees C and upon shifts between these two temperatures. The duration of the cell cycle in cells grown under continuous illumination at 20 degrees C was more than double that at 30 degrees C, suggesting that it was set directly by the growth rate. Similarly, the amounts of DNA, RNA, and bulk protein content per cell at 20 degrees C were approximately double those of cells grown at the higher temperature. For the shift experiments, cells grown at either 20 degrees C or 30 degrees C were transferred to darkness to prevent further growth, and then cultivated at the same or the other temperature. Upon transfer to the lower temperature, fewer nuclei and daughter cells were produced, and not all cells were able to finish the cell cycle by division, remaining multinuclear. Correspondingly, cells placed in the dark at the higher temperature divided faster into more daughter cells than the control cells. These differences correlated with shifts in the preceding cyclin-dependent kinase activity, suggesting that cell cycle progression was not related to growth rate or cell biomass but correlated with cyclin-dependent kinase activity.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Experimental Botany

ISSN

0022-0957

e-ISSN

—

Svazek periodika

70

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

14

Strana od-do

845-858

Kód UT WoS článku

000459350700012

EID výsledku v databázi Scopus

2-s2.0-85061118421