Cell Cycle Arrest by Supraoptimal Temperature in the Alga Chlamydomonas reinhardtii

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F19%3A00518103" target="_blank" >RIV/61388971:_____/19:00518103 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12310/19:43899782

Výsledek na webu

<a href="https://www.mdpi.com/2073-4409/8/10/1237" target="_blank" >https://www.mdpi.com/2073-4409/8/10/1237</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/cells8101237" target="_blank" >10.3390/cells8101237</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Cell Cycle Arrest by Supraoptimal Temperature in the Alga Chlamydomonas reinhardtii

Popis výsledku v původním jazyce

Temperature is one of the key factors affecting growth and division of algal cells. High temperature inhibits the cell cycle in Chlamydomonas reinhardtii. At 39 degrees C, nuclear and cellular divisions in synchronized cultures were blocked completely, while DNA replication was partly affected. In contrast, growth (cell volume, dry matter, total protein, and RNA) remained unaffected, and starch accumulated at very high levels. The cell cycle arrest could be removed by transfer to 30 degrees C, but a full recovery occurred only in cultures cultivated up to 14 h at 39 degrees C. Thereafter, individual cell cycle processes began to be affected in sequence, daughter cell release, cell division, and DNA replication. Cell cycle arrest was accompanied by high mitotic cyclin-dependent kinase activity that decreased after completion of nuclear and cellular division following transfer to 30 degrees C. Cell cycle arrest was, therefore, not caused by a lack of cyclin-dependent kinase activity but rather a blockage in downstream processes.

Název v anglickém jazyce

Cell Cycle Arrest by Supraoptimal Temperature in the Alga Chlamydomonas reinhardtii

Popis výsledku anglicky

Temperature is one of the key factors affecting growth and division of algal cells. High temperature inhibits the cell cycle in Chlamydomonas reinhardtii. At 39 degrees C, nuclear and cellular divisions in synchronized cultures were blocked completely, while DNA replication was partly affected. In contrast, growth (cell volume, dry matter, total protein, and RNA) remained unaffected, and starch accumulated at very high levels. The cell cycle arrest could be removed by transfer to 30 degrees C, but a full recovery occurred only in cultures cultivated up to 14 h at 39 degrees C. Thereafter, individual cell cycle processes began to be affected in sequence, daughter cell release, cell division, and DNA replication. Cell cycle arrest was accompanied by high mitotic cyclin-dependent kinase activity that decreased after completion of nuclear and cellular division following transfer to 30 degrees C. Cell cycle arrest was, therefore, not caused by a lack of cyclin-dependent kinase activity but rather a blockage in downstream processes.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10601 - Cell biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cells

ISSN

2073-4409

e-ISSN

—

Svazek periodika

8

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

18

Strana od-do

1237

Kód UT WoS článku

000497336400126

EID výsledku v databázi Scopus

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