In vitro response of human ovarian cancer cells to dietary bioflavonoid isoquercitrin

Kód výsledku v IS VaVaI

RIV/61388971:_____/19:00517769 - isvavai.cz

Výsledek na webu

https://www.tandfonline.com/doi/full/10.1080/03601234.2019.1633214

DOI - Digital Object Identifier

10.1080/03601234.2019.1633214

Jazyk výsledku

angličtina

Název v původním jazyce

In vitro response of human ovarian cancer cells to dietary bioflavonoid isoquercitrin

Popis výsledku v původním jazyce

Isoquercitrin is a dietary bioflavonoid used as a food supplement. We studied the mechanism underlying its effect in human ovarian cancer cells using OVCAR-3 cell line. Viability, survival, apoptosis, release of human transforming growth factor-beta 1 (TGF-beta 1) and TGF-beta 1 receptor, and intracellular reactive oxygen species (ROS) generation by OVCAR-3 cells were examined after isoquercitrin treatment at concentrations 5, 10, 25, 50, and 100 mu g mL(-1). AlamarBlue assay revealed that isoquercitrin did not cause any significant change (P > 0.05) in cell viability as compared to control. Apoptotic assay using flow cytometry did not find any significant change (P > 0.05) in the proportion of live, dead and apoptotic cells as compared to control. ELISA also showed that the release of human TGF-beta 1 and TGF-beta 1 receptor were not significantly (P > 0.05) affected by isoquercitrin as compared to control. Chemiluminescence assay demonstrated that lower concentrations (5, 10, and 25 mu g mL(-1)) were able to exhibit beneficial effects by inhibiting the generation of intracellular ROS. In contrast, elevated concentrations of 50 and 100 mu g mL(-1) led to oxidative stress (P < 0.05). We concluded that the beneficial effect of isoquercitrin on ovarian cancer cells may be mediated by an antioxidative pathway that involves inhibition of intracellular ROS generation, thereby limiting oxidative stress.

Název v anglickém jazyce

In vitro response of human ovarian cancer cells to dietary bioflavonoid isoquercitrin

Popis výsledku anglicky

Isoquercitrin is a dietary bioflavonoid used as a food supplement. We studied the mechanism underlying its effect in human ovarian cancer cells using OVCAR-3 cell line. Viability, survival, apoptosis, release of human transforming growth factor-beta 1 (TGF-beta 1) and TGF-beta 1 receptor, and intracellular reactive oxygen species (ROS) generation by OVCAR-3 cells were examined after isoquercitrin treatment at concentrations 5, 10, 25, 50, and 100 mu g mL(-1). AlamarBlue assay revealed that isoquercitrin did not cause any significant change (P > 0.05) in cell viability as compared to control. Apoptotic assay using flow cytometry did not find any significant change (P > 0.05) in the proportion of live, dead and apoptotic cells as compared to control. ELISA also showed that the release of human TGF-beta 1 and TGF-beta 1 receptor were not significantly (P > 0.05) affected by isoquercitrin as compared to control. Chemiluminescence assay demonstrated that lower concentrations (5, 10, and 25 mu g mL(-1)) were able to exhibit beneficial effects by inhibiting the generation of intracellular ROS. In contrast, elevated concentrations of 50 and 100 mu g mL(-1) led to oxidative stress (P < 0.05). We concluded that the beneficial effect of isoquercitrin on ovarian cancer cells may be mediated by an antioxidative pathway that involves inhibition of intracellular ROS generation, thereby limiting oxidative stress.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

GA18-00150S: Minoritní a neobvyklé flavonolignany: Chemo-enzymatická příprava a farmakologická aktivita

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Environmental Science and Health Part B-Pesticides Food Contaminants and Agricultural Wastes

ISSN

0360-1234

e-ISSN

—

Svazek periodika

54

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

6

Strana od-do

752-757

Kód UT WoS článku

000475047100001

EID výsledku v databázi Scopus

2-s2.0-85068624859