Rapid screening test to estimate temperature optima for microalgae growth using photosynthesis activity measurements

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F19%3A00517983" target="_blank" >RIV/61388971:_____/19:00517983 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12220/19:43899774 RIV/60076658:12310/19:43899774

Výsledek na webu

<a href="https://link.springer.com/article/10.1007%2Fs12223-019-00738-8" target="_blank" >https://link.springer.com/article/10.1007%2Fs12223-019-00738-8</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s12223-019-00738-8" target="_blank" >10.1007/s12223-019-00738-8</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Rapid screening test to estimate temperature optima for microalgae growth using photosynthesis activity measurements

Popis výsledku v původním jazyce

We have worked out a rapid 1-day test based on photosynthesis measurements to estimate suitable growth temperature of microalgae cultures. To verify the proposed procedure, several microalgae-Chlorella, Nostoc, Synechocystis, Scenedesmus, and Cylindrospermum-were cultured under controlled laboratory conditions (irradiance, temperature, mixing, CO2, and nutrient supply) to find the optima of photosynthetic activity using the range between 15 and 35 degrees C. These activities were recorded at each temperature step after 2 h of acclimation which should be sufficient as oxygen production and the PQ cycle are regulated by fast processes. Photosynthetic activity was measured using three techniques-oxygen production/respiration, saturating pulse analysis of fluorescence quenching, and fast fluorescence induction kinetics-to estimate the temperature optima which should correspond to high growth rate. We measured all variables that might have been directly related to growth-photosynthetic oxygen evolution, maximum photochemical yield of PSII, F-v/F-m, relative electron transport rate rETR(max), and the transients V-j and V-i determined by fast fluorescence induction curves. When the temperature optima for photosynthetic activity were verified in growth tests, we found good correlation. For most of tested microalgae strains, temperature around 30 degrees C was found to be the most suitable at this setting. We concluded that the developed test can be used as a rapid 1-day pre-screening to estimate a suitable growth temperature of microalgae strains before they are cultured in a pilot scale.

Název v anglickém jazyce

Rapid screening test to estimate temperature optima for microalgae growth using photosynthesis activity measurements

Popis výsledku anglicky

We have worked out a rapid 1-day test based on photosynthesis measurements to estimate suitable growth temperature of microalgae cultures. To verify the proposed procedure, several microalgae-Chlorella, Nostoc, Synechocystis, Scenedesmus, and Cylindrospermum-were cultured under controlled laboratory conditions (irradiance, temperature, mixing, CO2, and nutrient supply) to find the optima of photosynthetic activity using the range between 15 and 35 degrees C. These activities were recorded at each temperature step after 2 h of acclimation which should be sufficient as oxygen production and the PQ cycle are regulated by fast processes. Photosynthetic activity was measured using three techniques-oxygen production/respiration, saturating pulse analysis of fluorescence quenching, and fast fluorescence induction kinetics-to estimate the temperature optima which should correspond to high growth rate. We measured all variables that might have been directly related to growth-photosynthetic oxygen evolution, maximum photochemical yield of PSII, F-v/F-m, relative electron transport rate rETR(max), and the transients V-j and V-i determined by fast fluorescence induction curves. When the temperature optima for photosynthetic activity were verified in growth tests, we found good correlation. For most of tested microalgae strains, temperature around 30 degrees C was found to be the most suitable at this setting. We concluded that the developed test can be used as a rapid 1-day pre-screening to estimate a suitable growth temperature of microalgae strains before they are cultured in a pilot scale.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/LO1416" target="_blank" >LO1416: Algatech plus</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Folia Microbiologica

ISSN

0015-5632

e-ISSN

—

Svazek periodika

64

Číslo periodika v rámci svazku

5 SI

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

11

Strana od-do

615-625

Kód UT WoS článku

000496409500003

EID výsledku v databázi Scopus

2-s2.0-85069902262