Photosynthetic monitoring techniques indicate maximum glycogen accumulation in nitrogen-limited Synechocystis sp. PCC 6803 culture

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12220%2F21%3A43903324" target="_blank" >RIV/60076658:12220/21:43903324 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12310/21:43903324 RIV/61388971:_____/21:00543898

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S2211926421000904?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S2211926421000904?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.algal.2021.102271" target="_blank" >10.1016/j.algal.2021.102271</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Photosynthetic monitoring techniques indicate maximum glycogen accumulation in nitrogen-limited Synechocystis sp. PCC 6803 culture

Popis výsledku v původním jazyce

Chlorophyll fluorescence and oxygen evolution are rapid and non-invasive monitoring techniques to obtain information about the photosynthesis performance of microalgae cultures. These methods may be employed for optimizing the biomass productivity as well as indicate increased production of some valuable compounds. In this work photosynthesis monitoring techniques ? saturation pulse analysis of fluorescence quenching, fast fluorescence induction kinetics and photosynthetic oxygen production/respiration ? were employed for monitoring of the physiological state of the nitrogen-limited culture of Synechocystis sp. PCC 6803. The correlation between changes of photosynthetic activities, growth and glycogen accumulation was examined in these cultures. The aim was to determine proper point for biomass harvest comparing the changes of photosynthetic activities and the kinetics of glycogen accumulation. In a 4-day trial the highest glycogen accumulation in biomass was found on day 2 showing an inflection point which was accompanied by the reversal of the chlorophyll fluorescence variables, namely maximal photochemical efficiency of PSII Fv/Fm, relative electron transport rate rETR, photochemical efficiency ? and photosynthesis saturating irradiance Ik. The rapid increase of cell respiration after day 2 also indicated the relation of these changes with onset of glycogen catabolism. Our laboratory experiments revealed that the three photosynthetic monitoring techniques provided good indication of the physiological changes leading to high glycogen accumulation in biomass of the nitrogen-limited Synechocystis culture. In this way the glycogen accumulation can be manipulated. The photosynthesis measurements can indicate the right time to harvest the culture rich in glycogen in biotechnological applications.

Název v anglickém jazyce

Photosynthetic monitoring techniques indicate maximum glycogen accumulation in nitrogen-limited Synechocystis sp. PCC 6803 culture

Popis výsledku anglicky

Chlorophyll fluorescence and oxygen evolution are rapid and non-invasive monitoring techniques to obtain information about the photosynthesis performance of microalgae cultures. These methods may be employed for optimizing the biomass productivity as well as indicate increased production of some valuable compounds. In this work photosynthesis monitoring techniques ? saturation pulse analysis of fluorescence quenching, fast fluorescence induction kinetics and photosynthetic oxygen production/respiration ? were employed for monitoring of the physiological state of the nitrogen-limited culture of Synechocystis sp. PCC 6803. The correlation between changes of photosynthetic activities, growth and glycogen accumulation was examined in these cultures. The aim was to determine proper point for biomass harvest comparing the changes of photosynthetic activities and the kinetics of glycogen accumulation. In a 4-day trial the highest glycogen accumulation in biomass was found on day 2 showing an inflection point which was accompanied by the reversal of the chlorophyll fluorescence variables, namely maximal photochemical efficiency of PSII Fv/Fm, relative electron transport rate rETR, photochemical efficiency ? and photosynthesis saturating irradiance Ik. The rapid increase of cell respiration after day 2 also indicated the relation of these changes with onset of glycogen catabolism. Our laboratory experiments revealed that the three photosynthetic monitoring techniques provided good indication of the physiological changes leading to high glycogen accumulation in biomass of the nitrogen-limited Synechocystis culture. In this way the glycogen accumulation can be manipulated. The photosynthesis measurements can indicate the right time to harvest the culture rich in glycogen in biotechnological applications.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Algal Research-Biomass Biofuels and Bioproducts

ISSN

2211-9264

e-ISSN

—

Svazek periodika

55

Číslo periodika v rámci svazku

MAY 2021

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

—

Kód UT WoS článku

000642454600005

EID výsledku v databázi Scopus

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