Rapid screening of very long-chain fatty acids from microorganisms
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F19%3A00518107" target="_blank" >RIV/61388971:_____/19:00518107 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/67985939:_____/19:00518107 RIV/00216208:11310/19:10409537 RIV/60461373:22330/19:43918587
Výsledek na webu
<a href="https://www.sciencedirect.com/science/article/pii/S0021967319307496?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0021967319307496?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.chroma.2019.460365" target="_blank" >10.1016/j.chroma.2019.460365</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Rapid screening of very long-chain fatty acids from microorganisms
Popis výsledku v původním jazyce
The analysis of triacylglycerols and phospholipids - phosphatidylcholines allowed the use of shotgun lipidomics to identify very long-chain fatty acids and very long-chain polyunsaturated fatty acids in microalgae. These fatty acids were determined in triacylglycerols by positive electrospray ionization of neutral loss scans of different fatty acids, e.g. 24:0, 24:19, 1 omega 9, 24:6 omega 3, 26:0, 26:1 omega 9, 28:0, 28:1 omega 9, 28:2 omega 6 and 28:8 omega 3. Likewise, very long-chain fatty acids in phosphatidylcholines were identified by negative electrospray ionization mass spectrometry in the selected ion-monitoring of the two most important ions (R1COO- and R2COO-). The limit of detection was determined at 10 nmol/L (similar to 11 pg/mu L) in triacylglycerols and 8.6 nmoles/L (similar to 8 pg/mu L) in phosphatidylcholines. The use of liquid chromatography-mass spectrometry is suitable for very long-chain polyunsaturated fatty acids with up to 8 double bonds due to the time of analysis as well as for reasons of lower thermal stability of polyunsaturated fatty acids towards saturated fatty acids, but gas chromatography-mass spectrometry is better suited for the analysis of saturated very long-chain fatty acids
Název v anglickém jazyce
Rapid screening of very long-chain fatty acids from microorganisms
Popis výsledku anglicky
The analysis of triacylglycerols and phospholipids - phosphatidylcholines allowed the use of shotgun lipidomics to identify very long-chain fatty acids and very long-chain polyunsaturated fatty acids in microalgae. These fatty acids were determined in triacylglycerols by positive electrospray ionization of neutral loss scans of different fatty acids, e.g. 24:0, 24:19, 1 omega 9, 24:6 omega 3, 26:0, 26:1 omega 9, 28:0, 28:1 omega 9, 28:2 omega 6 and 28:8 omega 3. Likewise, very long-chain fatty acids in phosphatidylcholines were identified by negative electrospray ionization mass spectrometry in the selected ion-monitoring of the two most important ions (R1COO- and R2COO-). The limit of detection was determined at 10 nmol/L (similar to 11 pg/mu L) in triacylglycerols and 8.6 nmoles/L (similar to 8 pg/mu L) in phosphatidylcholines. The use of liquid chromatography-mass spectrometry is suitable for very long-chain polyunsaturated fatty acids with up to 8 double bonds due to the time of analysis as well as for reasons of lower thermal stability of polyunsaturated fatty acids towards saturated fatty acids, but gas chromatography-mass spectrometry is better suited for the analysis of saturated very long-chain fatty acids
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10606 - Microbiology
Návaznosti výsledku
Projekt
<a href="/cs/project/GA17-00027S" target="_blank" >GA17-00027S: Velmi dlouhé mastné kyseliny z mikroorganizmů</a><br>
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2019
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Journal of Chromatography A
ISSN
0021-9673
e-ISSN
—
Svazek periodika
1605
Číslo periodika v rámci svazku
NOV 8 2019
Stát vydavatele periodika
NL - Nizozemsko
Počet stran výsledku
10
Strana od-do
UNSP 460365
Kód UT WoS článku
000500389200017
EID výsledku v databázi Scopus
2-s2.0-85070227846