Rapid screening of very long-chain fatty acids from microorganisms

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F19%3A00518107" target="_blank" >RIV/61388971:_____/19:00518107 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985939:_____/19:00518107 RIV/00216208:11310/19:10409537 RIV/60461373:22330/19:43918587

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0021967319307496?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0021967319307496?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.chroma.2019.460365" target="_blank" >10.1016/j.chroma.2019.460365</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Rapid screening of very long-chain fatty acids from microorganisms

Popis výsledku v původním jazyce

The analysis of triacylglycerols and phospholipids - phosphatidylcholines allowed the use of shotgun lipidomics to identify very long-chain fatty acids and very long-chain polyunsaturated fatty acids in microalgae. These fatty acids were determined in triacylglycerols by positive electrospray ionization of neutral loss scans of different fatty acids, e.g. 24:0, 24:19, 1 omega 9, 24:6 omega 3, 26:0, 26:1 omega 9, 28:0, 28:1 omega 9, 28:2 omega 6 and 28:8 omega 3. Likewise, very long-chain fatty acids in phosphatidylcholines were identified by negative electrospray ionization mass spectrometry in the selected ion-monitoring of the two most important ions (R1COO- and R2COO-). The limit of detection was determined at 10 nmol/L (similar to 11 pg/mu L) in triacylglycerols and 8.6 nmoles/L (similar to 8 pg/mu L) in phosphatidylcholines. The use of liquid chromatography-mass spectrometry is suitable for very long-chain polyunsaturated fatty acids with up to 8 double bonds due to the time of analysis as well as for reasons of lower thermal stability of polyunsaturated fatty acids towards saturated fatty acids, but gas chromatography-mass spectrometry is better suited for the analysis of saturated very long-chain fatty acids

Název v anglickém jazyce

Rapid screening of very long-chain fatty acids from microorganisms

Popis výsledku anglicky

The analysis of triacylglycerols and phospholipids - phosphatidylcholines allowed the use of shotgun lipidomics to identify very long-chain fatty acids and very long-chain polyunsaturated fatty acids in microalgae. These fatty acids were determined in triacylglycerols by positive electrospray ionization of neutral loss scans of different fatty acids, e.g. 24:0, 24:19, 1 omega 9, 24:6 omega 3, 26:0, 26:1 omega 9, 28:0, 28:1 omega 9, 28:2 omega 6 and 28:8 omega 3. Likewise, very long-chain fatty acids in phosphatidylcholines were identified by negative electrospray ionization mass spectrometry in the selected ion-monitoring of the two most important ions (R1COO- and R2COO-). The limit of detection was determined at 10 nmol/L (similar to 11 pg/mu L) in triacylglycerols and 8.6 nmoles/L (similar to 8 pg/mu L) in phosphatidylcholines. The use of liquid chromatography-mass spectrometry is suitable for very long-chain polyunsaturated fatty acids with up to 8 double bonds due to the time of analysis as well as for reasons of lower thermal stability of polyunsaturated fatty acids towards saturated fatty acids, but gas chromatography-mass spectrometry is better suited for the analysis of saturated very long-chain fatty acids

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/GA17-00027S" target="_blank" >GA17-00027S: Velmi dlouhé mastné kyseliny z mikroorganizmů</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Chromatography A

ISSN

0021-9673

e-ISSN

—

Svazek periodika

1605

Číslo periodika v rámci svazku

NOV 8 2019

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

10

Strana od-do

UNSP 460365

Kód UT WoS článku

000500389200017

EID výsledku v databázi Scopus

2-s2.0-85070227846