Chlorophyll f synthesis by a super-rogue photosystem II complex

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F20%3A00524548" target="_blank" >RIV/61388971:_____/20:00524548 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.nature.com/articles/s41477-020-0616-4" target="_blank" >https://www.nature.com/articles/s41477-020-0616-4</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41477-020-0616-4" target="_blank" >10.1038/s41477-020-0616-4</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Chlorophyll f synthesis by a super-rogue photosystem II complex

Popis výsledku v původním jazyce

Certain cyanobacteria synthesize chlorophyll molecules (Chl d and Chl f) that absorb in the far-red region of the solar spectrum, thereby extending the spectral range of photosynthetically active radiation(1,2). The synthesis and introduction of these far-red chlorophylls into the photosynthetic apparatus of plants might improve the efficiency of oxygenic photosynthesis, especially in far-red enriched environments, such as in the lower regions of the canopy(3). Production of Chl f requires the ChlF subunit, also known as PsbA4 (ref. (4)) or super-rogue D1 (ref. (5)), a paralogue of the D1 subunit of photosystem II (PSII) which, together with D2, bind cofactors involved in the light-driven oxidation of water. Current ideas suggest that ChlF oxidizes Chl a to Chl f in a homodimeric ChlF reaction centre (RC) complex and represents a missing link in the evolution of the heterodimeric D1/D2 RC of PSII (refs. (4,6)). However, unambiguous biochemical support for this proposal is lacking. Here, we show that ChlF can substitute for D1 to form modified PSII complexes capable of producing Chl f. Remarkably, mutation of just two residues in D1 converts oxygen-evolving PSII into a Chl f synthase. Overall, we have identified a new class of PSII complex, which we term 'super-rogue' PSII, with an unexpected role in pigment biosynthesis rather than water oxidation.nThe cyanobacterial chlorophyll, Chl f, absorbs far-red light. Mutation of two residues in a subunit of photosystem II converts it to a Chl f synthase. This 'super-rogue' photosystem might improve photosynthetic efficiency in low light.

Název v anglickém jazyce

Chlorophyll f synthesis by a super-rogue photosystem II complex

Popis výsledku anglicky

Certain cyanobacteria synthesize chlorophyll molecules (Chl d and Chl f) that absorb in the far-red region of the solar spectrum, thereby extending the spectral range of photosynthetically active radiation(1,2). The synthesis and introduction of these far-red chlorophylls into the photosynthetic apparatus of plants might improve the efficiency of oxygenic photosynthesis, especially in far-red enriched environments, such as in the lower regions of the canopy(3). Production of Chl f requires the ChlF subunit, also known as PsbA4 (ref. (4)) or super-rogue D1 (ref. (5)), a paralogue of the D1 subunit of photosystem II (PSII) which, together with D2, bind cofactors involved in the light-driven oxidation of water. Current ideas suggest that ChlF oxidizes Chl a to Chl f in a homodimeric ChlF reaction centre (RC) complex and represents a missing link in the evolution of the heterodimeric D1/D2 RC of PSII (refs. (4,6)). However, unambiguous biochemical support for this proposal is lacking. Here, we show that ChlF can substitute for D1 to form modified PSII complexes capable of producing Chl f. Remarkably, mutation of just two residues in D1 converts oxygen-evolving PSII into a Chl f synthase. Overall, we have identified a new class of PSII complex, which we term 'super-rogue' PSII, with an unexpected role in pigment biosynthesis rather than water oxidation.nThe cyanobacterial chlorophyll, Chl f, absorbs far-red light. Mutation of two residues in a subunit of photosystem II converts it to a Chl f synthase. This 'super-rogue' photosystem might improve photosynthetic efficiency in low light.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Nature Plants

ISSN

2055-026X

e-ISSN

—

Svazek periodika

6

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

7

Strana od-do

238-244

Kód UT WoS článku

000519577200015

EID výsledku v databázi Scopus

2-s2.0-85081990201