Insights from enzymatic degradation of cellulose and hemicellulose to fermentable sugars- a review

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F20%3A00525065" target="_blank" >RIV/61388971:_____/20:00525065 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0961953420300155" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0961953420300155</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.biombioe.2020.105481" target="_blank" >10.1016/j.biombioe.2020.105481</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Insights from enzymatic degradation of cellulose and hemicellulose to fermentable sugars- a review

Popis výsledku v původním jazyce

Lignocellulose, the most abundant and renewable resource on Earth is an important raw material, which can be converted into high value products. However, to this end, it needs to be pretreated physically, chemically, or biologically. Its complex structure and recalcitrance against physical, chemical, or biological degradation render its breakdown an important target of study. The understanding of the enzymatic processes of lignocellulose breakdown and the changes in its chemistry are thus essential. Here, we review the current analytical challenges in the analysis of lignocellulose composition, lignocelluloytic pretreatment, analysis of enzymatic hydrolysis catalyzed by cellulases or hemicellulases and their biotechnological applications.nComplex techniques including biochemical, genomic, and metagenomics methods such as high performance anion exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD), Respiration Activity Monitoring System (RAMOS), and next-generation sequencing are described. HPAEC-PAD is a promising, rapid, and reliable analytical technique for sugar quantification following lignocellulose breakdown. RAMOS is an effective technique for monitoring the growth of microorganisms during the different phases of enzyme production, enzymatic hydrolysis, and fermentation. The emergence of high throughput, next-generation sequencing techniques has enriched the databases of genes encoding glycoside hydrolase classes commonly involved in lignocellulose decomposition, and this knowledge can be readily used to analyse the involved processes. Still, novel analytical methods are highly welcome to understand the complete process of lignocelluloytic breakdown. In order to decrease environmental pollution and to save energy, lignocellulose conversion needs to be promoted in order to effectively compete with fossil resources on a global scale in future.

Název v anglickém jazyce

Insights from enzymatic degradation of cellulose and hemicellulose to fermentable sugars- a review

Popis výsledku anglicky

Lignocellulose, the most abundant and renewable resource on Earth is an important raw material, which can be converted into high value products. However, to this end, it needs to be pretreated physically, chemically, or biologically. Its complex structure and recalcitrance against physical, chemical, or biological degradation render its breakdown an important target of study. The understanding of the enzymatic processes of lignocellulose breakdown and the changes in its chemistry are thus essential. Here, we review the current analytical challenges in the analysis of lignocellulose composition, lignocelluloytic pretreatment, analysis of enzymatic hydrolysis catalyzed by cellulases or hemicellulases and their biotechnological applications.nComplex techniques including biochemical, genomic, and metagenomics methods such as high performance anion exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD), Respiration Activity Monitoring System (RAMOS), and next-generation sequencing are described. HPAEC-PAD is a promising, rapid, and reliable analytical technique for sugar quantification following lignocellulose breakdown. RAMOS is an effective technique for monitoring the growth of microorganisms during the different phases of enzyme production, enzymatic hydrolysis, and fermentation. The emergence of high throughput, next-generation sequencing techniques has enriched the databases of genes encoding glycoside hydrolase classes commonly involved in lignocellulose decomposition, and this knowledge can be readily used to analyse the involved processes. Still, novel analytical methods are highly welcome to understand the complete process of lignocelluloytic breakdown. In order to decrease environmental pollution and to save energy, lignocellulose conversion needs to be promoted in order to effectively compete with fossil resources on a global scale in future.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biomass & Bioenergy

ISSN

0961-9534

e-ISSN

—

Svazek periodika

134

Číslo periodika v rámci svazku

MAR

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

13

Strana od-do

—

Kód UT WoS článku

000517497400018

EID výsledku v databázi Scopus

2-s2.0-85078758628