Immunoglobulin light chain kappa precedes Lambda rearrangement in swine but a majority of Lambda+ B cells are generated earlier

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F20%3A00532773" target="_blank" >RIV/61388971:_____/20:00532773 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0145305X20301749" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0145305X20301749</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.dci.2020.103751" target="_blank" >10.1016/j.dci.2020.103751</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Immunoglobulin light chain kappa precedes Lambda rearrangement in swine but a majority of Lambda+ B cells are generated earlier

Popis výsledku v původním jazyce

Developmental pathways for B cell lymphogenesis are sufficiently known only in mice and humans. However, both of these species rearrange immunoglobulin heavy chains (IgH) before light chains (IgL) while IgL precedes IgH rearrangement in swine. We demonstrate here that this reversed order of rearrangements have some concealed consequences: (1) we confirmed that although IgLκ rearrangement is initial, most IgLλ+ B cells are generated earlier and before IgH rearrangements, while most IgLκ+ B cells later and after IgH rearrangements, (2) the second IgLκ rearrangement can occur after IgLλ rearrangement, (3) early formed B cells bear only single in-frame IgH rearrangements, (4) many IgLκ+ B cells carry IgLλ rearrangements that can be productive and occurring on both alleles in one cell, and (5) although VpreB and λ5 genes are present in swine, they are preferentially expressed in non-B cells. In summary, our findings reveal that swine use an alternative B cell developmental pathway as compared to mice and humans.nn

Název v anglickém jazyce

Immunoglobulin light chain kappa precedes Lambda rearrangement in swine but a majority of Lambda+ B cells are generated earlier

Popis výsledku anglicky

Developmental pathways for B cell lymphogenesis are sufficiently known only in mice and humans. However, both of these species rearrange immunoglobulin heavy chains (IgH) before light chains (IgL) while IgL precedes IgH rearrangement in swine. We demonstrate here that this reversed order of rearrangements have some concealed consequences: (1) we confirmed that although IgLκ rearrangement is initial, most IgLλ+ B cells are generated earlier and before IgH rearrangements, while most IgLκ+ B cells later and after IgH rearrangements, (2) the second IgLκ rearrangement can occur after IgLλ rearrangement, (3) early formed B cells bear only single in-frame IgH rearrangements, (4) many IgLκ+ B cells carry IgLλ rearrangements that can be productive and occurring on both alleles in one cell, and (5) although VpreB and λ5 genes are present in swine, they are preferentially expressed in non-B cells. In summary, our findings reveal that swine use an alternative B cell developmental pathway as compared to mice and humans.nn

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10613 - Zoology

Projekt

<a href="/cs/project/GA19-01504S" target="_blank" >GA19-01504S: Odlišná posloupnost přeskupování lehkých řetězců protilátek u prasat</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Developmental and Comparative Immunology

ISSN

0145-305X

e-ISSN

—

Svazek periodika

111

Číslo periodika v rámci svazku

October 2020

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

10

Strana od-do

103751

Kód UT WoS článku

000551873700009

EID výsledku v databázi Scopus

2-s2.0-85086453527