Consequences of the different order of immunoglobulin gene rearrangements in swine

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F22%3A00550862" target="_blank" >RIV/61388971:_____/22:00550862 - isvavai.cz</a>

Výsledek na webu

<a href="https://www-sciencedirect-com.d360prx.biomed.cas.cz/science/article/pii/S0145305X21002044" target="_blank" >https://www-sciencedirect-com.d360prx.biomed.cas.cz/science/article/pii/S0145305X21002044</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.dci.2021.104196" target="_blank" >10.1016/j.dci.2021.104196</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Consequences of the different order of immunoglobulin gene rearrangements in swine

Popis výsledku v původním jazyce

Swine use a reverse order of immunoglobulin chain rearrangement compared to humans and mice, and this altered and modified order should have measurable consequences. Here we perform new and defining experiments with developing and mature B cells, characterizing the B cell populations that do not exist in other species. First, we have finally confirmed that light chains kappa and lambda are rearranged and expressed on the surface before any heavy chain rearrangements using western-blot. And second, we have analyzed a pool of mature B cells on the single-cell level to demonstrate that many kappa+ mature B cells carry lambda transcripts. According to these findings, we believe that there may be more groups of mammals, one of which uses a pre-BCR-driven developmental pathway for B cell generation (like mice and humans), the second group uses a pre-BCR-independent one (like swine), and some may be even intermediate.

Název v anglickém jazyce

Consequences of the different order of immunoglobulin gene rearrangements in swine

Popis výsledku anglicky

Swine use a reverse order of immunoglobulin chain rearrangement compared to humans and mice, and this altered and modified order should have measurable consequences. Here we perform new and defining experiments with developing and mature B cells, characterizing the B cell populations that do not exist in other species. First, we have finally confirmed that light chains kappa and lambda are rearranged and expressed on the surface before any heavy chain rearrangements using western-blot. And second, we have analyzed a pool of mature B cells on the single-cell level to demonstrate that many kappa+ mature B cells carry lambda transcripts. According to these findings, we believe that there may be more groups of mammals, one of which uses a pre-BCR-driven developmental pathway for B cell generation (like mice and humans), the second group uses a pre-BCR-independent one (like swine), and some may be even intermediate.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10613 - Zoology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Developmental and Comparative Immunology

ISSN

0145-305X

e-ISSN

1879-0089

Svazek periodika

126

Číslo periodika v rámci svazku

JAN 2022

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

7

Strana od-do

104196

Kód UT WoS článku

000703565600001

EID výsledku v databázi Scopus

2-s2.0-85114184162