Comparative Aspects of Immunoglobulin Gene Rearrangement Arrays in Different Species

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F22%3A00553320" target="_blank" >RIV/61388971:_____/22:00553320 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.frontiersin.org/articles/10.3389/fimmu.2022.823145/full" target="_blank" >https://www.frontiersin.org/articles/10.3389/fimmu.2022.823145/full</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fimmu.2022.823145" target="_blank" >10.3389/fimmu.2022.823145</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Comparative Aspects of Immunoglobulin Gene Rearrangement Arrays in Different Species

Popis výsledku v původním jazyce

Studies in humans and mice indicate the critical role of the surrogate light chain in thenselection of the productive immunoglobulin repertoire during B cell development.nHowever, subsequent studies using mutant mice have also demonstrated thatnalternative pathways are allowed. Our recent investigation has shown that somenspecies, such as pig, physiologically use preferential rearrangement of authentic lightnchains, and become independent of surrogate light chains. Here we summarize thenfindings from swine and compare them with results in other species. In both groups, allelicnand isotypic exclusions remain intact, so the different processes do not alter the paradigmnof B-cell monospecificity. Both groups also retained some other essential processes, suchnas segregated and sequential rearrangement of heavy and light chain loci, preferentialnrearrangement of light chain kappa before lambda, and functional k-deleting elementnrecombination. On the other hand, the respective order of heavy and light chainsnrearrangement may vary, and rearrangement of the light chain kappa and lambda onndifferent chromosomes may occur independently. Studies have also confirmed that thensurrogate light chain is not required for the selection of the productive repertoire of heavynchains and can be substituted by authentic light chains. These findings are important fornunderstanding evolutional approaches, redundancy and efficiency of B-cell generation,ndependencies on other regulatory factors, and strategies for constructing therapeuticnantibodies in unrelated species. The results may also be important for explainingninterspecies differences in the proportional use of light chains and for the understandingnof divergences in rearrangement processes. Therefore, the division into two groups maynnot be definitive and there may be more groups of intermediate species.

Název v anglickém jazyce

Comparative Aspects of Immunoglobulin Gene Rearrangement Arrays in Different Species

Popis výsledku anglicky

Studies in humans and mice indicate the critical role of the surrogate light chain in thenselection of the productive immunoglobulin repertoire during B cell development.nHowever, subsequent studies using mutant mice have also demonstrated thatnalternative pathways are allowed. Our recent investigation has shown that somenspecies, such as pig, physiologically use preferential rearrangement of authentic lightnchains, and become independent of surrogate light chains. Here we summarize thenfindings from swine and compare them with results in other species. In both groups, allelicnand isotypic exclusions remain intact, so the different processes do not alter the paradigmnof B-cell monospecificity. Both groups also retained some other essential processes, suchnas segregated and sequential rearrangement of heavy and light chain loci, preferentialnrearrangement of light chain kappa before lambda, and functional k-deleting elementnrecombination. On the other hand, the respective order of heavy and light chainsnrearrangement may vary, and rearrangement of the light chain kappa and lambda onndifferent chromosomes may occur independently. Studies have also confirmed that thensurrogate light chain is not required for the selection of the productive repertoire of heavynchains and can be substituted by authentic light chains. These findings are important fornunderstanding evolutional approaches, redundancy and efficiency of B-cell generation,ndependencies on other regulatory factors, and strategies for constructing therapeuticnantibodies in unrelated species. The results may also be important for explainingninterspecies differences in the proportional use of light chains and for the understandingnof divergences in rearrangement processes. Therefore, the division into two groups maynnot be definitive and there may be more groups of intermediate species.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30102 - Immunology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Frontiers in Immunology

ISSN

1664-3224

e-ISSN

1664-3224

Svazek periodika

13

Číslo periodika v rámci svazku

February 2022

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

11

Strana od-do

823145

Kód UT WoS článku

000761446800001

EID výsledku v databázi Scopus

2-s2.0-85125246124