Feeding on fungi: genomic and proteomic analysis of the enzymatic machinery of bacteria decomposing fungal biomass

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F20%3A00535741" target="_blank" >RIV/61388971:_____/20:00535741 - isvavai.cz</a>

Výsledek na webu

<a href="https://sfamjournals.onlinelibrary.wiley.com/doi/abs/10.1111/1462-2920.15183" target="_blank" >https://sfamjournals.onlinelibrary.wiley.com/doi/abs/10.1111/1462-2920.15183</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/1462-2920.15183" target="_blank" >10.1111/1462-2920.15183</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Feeding on fungi: genomic and proteomic analysis of the enzymatic machinery of bacteria decomposing fungal biomass

Popis výsledku v původním jazyce

Dead fungal biomass is an abundant source of nutrition in both litter and soil of temperate forests largely decomposed by bacteria. Here, we have examined the utilization of dead fungal biomass by the five dominant bacteria isolated from thein situdecomposition of fungal mycelia using a multiOMIC approach. The genomes of the isolates encoded a broad suite of carbohydrate-active enzymes, peptidases and transporters. In the extracellular proteome, onlyEwingella americanaexpressed chitinases while the twoPseudomonasisolates attacked chitin by lytic chitin monooxygenase, deacetylation and deamination.Variovoraxsp. expressed enzymes acting on the side-chains of various glucans and the chitin backbone. Surprisingly, despite its genomic potential,Pedobactersp. did not produce extracellular proteins to decompose fungal mycelia but presumably feeds on simple substrates. The ecological roles of the five individual strains exhibited complementary features for a fast and efficient decomposition of dead fungal biomass by the entire bacterial community.

Název v anglickém jazyce

Feeding on fungi: genomic and proteomic analysis of the enzymatic machinery of bacteria decomposing fungal biomass

Popis výsledku anglicky

Dead fungal biomass is an abundant source of nutrition in both litter and soil of temperate forests largely decomposed by bacteria. Here, we have examined the utilization of dead fungal biomass by the five dominant bacteria isolated from thein situdecomposition of fungal mycelia using a multiOMIC approach. The genomes of the isolates encoded a broad suite of carbohydrate-active enzymes, peptidases and transporters. In the extracellular proteome, onlyEwingella americanaexpressed chitinases while the twoPseudomonasisolates attacked chitin by lytic chitin monooxygenase, deacetylation and deamination.Variovoraxsp. expressed enzymes acting on the side-chains of various glucans and the chitin backbone. Surprisingly, despite its genomic potential,Pedobactersp. did not produce extracellular proteins to decompose fungal mycelia but presumably feeds on simple substrates. The ecological roles of the five individual strains exhibited complementary features for a fast and efficient decomposition of dead fungal biomass by the entire bacterial community.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Environmental Microbiology

ISSN

1462-2912

e-ISSN

—

Svazek periodika

22

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

16

Strana od-do

4604-4619

Kód UT WoS článku

000564015400001

EID výsledku v databázi Scopus

2-s2.0-85089996565