Bringing SEM and MSI closer than ever before: Visualizing aspergillus and pseudomonas infection in the rat lungs

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F20%3A00536784" target="_blank" >RIV/61388971:_____/20:00536784 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989592:15110/20:73602619 RIV/44555601:13440/20:43895624 RIV/00216208:11310/20:10421381

Výsledek na webu

<a href="https://www.mdpi.com/2309-608X/6/4/257" target="_blank" >https://www.mdpi.com/2309-608X/6/4/257</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/jof6040257" target="_blank" >10.3390/jof6040257</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Bringing SEM and MSI closer than ever before: Visualizing aspergillus and pseudomonas infection in the rat lungs

Popis výsledku v původním jazyce

A procedure for processing frozen rat lung tissue sections for scanning electron microscopy (SEM) from deeply frozen samples initially collected and stored for matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) was developed. The procedure employed slow thawing of the frozen sections while floating on the surface and melting in a fixative solution. After the float-washing step, the sections were dehydrated in a graded ethanol series and dried in a critical point dryer. The SEM generated images with well-preserved structures, allowing for monitoring of bacterial cells and fungal hyphae in the infected tissue. Importantly, the consecutive nonfixed frozen sections were fully compatible with MALDI-MSI, providing molecular biomarker maps of Pseudomonas aeruginosa. The protocol enables bimodal image fusion in the in-house software CycloBranch, as demonstrated by SEM and MALDI-MSI.

Název v anglickém jazyce

Bringing SEM and MSI closer than ever before: Visualizing aspergillus and pseudomonas infection in the rat lungs

Popis výsledku anglicky

A procedure for processing frozen rat lung tissue sections for scanning electron microscopy (SEM) from deeply frozen samples initially collected and stored for matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) was developed. The procedure employed slow thawing of the frozen sections while floating on the surface and melting in a fixative solution. After the float-washing step, the sections were dehydrated in a graded ethanol series and dried in a critical point dryer. The SEM generated images with well-preserved structures, allowing for monitoring of bacterial cells and fungal hyphae in the infected tissue. Importantly, the consecutive nonfixed frozen sections were fully compatible with MALDI-MSI, providing molecular biomarker maps of Pseudomonas aeruginosa. The protocol enables bimodal image fusion in the in-house software CycloBranch, as demonstrated by SEM and MALDI-MSI.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Fungi

ISSN

2309-608X

e-ISSN

—

Svazek periodika

6

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

12

Strana od-do

257

Kód UT WoS článku

000601657200001

EID výsledku v databázi Scopus

2-s2.0-85094863357