A biorefinery approach to obtain docosahexaenoic acid and docosapentaenoic acid n-6 from Schizochytrium using high performance countercurrent chromatography

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F21%3A00542764" target="_blank" >RIV/61388971:_____/21:00542764 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12310/21:43903452

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S2211926421000606?via%3Dihub#" target="_blank" >https://www.sciencedirect.com/science/article/pii/S2211926421000606?via%3Dihub#</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.algal.2021.102241" target="_blank" >10.1016/j.algal.2021.102241</a>

Jazyk výsledku

angličtina

Název v původním jazyce

A biorefinery approach to obtain docosahexaenoic acid and docosapentaenoic acid n-6 from Schizochytrium using high performance countercurrent chromatography

Popis výsledku v původním jazyce

Schizochytrium has arisen as a valuable alternative to fish as a source of polyunsaturated fatty acids. In the present study, Schizochytrium limacinum CO3-H biomass was processed by direct transesterification affording a transesterified algal oil, from which docosahexaenoic acid (DHA) and docosapentaenoic acid n-6 (DPA n-6) ethyl esters were isolated using high performance countercurrent chromatography (HPCCC). A multiple sequential injection HPCCC separation method was developed combining two elution modes (reverse phase and extrusion). During the reverse phase elution, the lower phase of a biphasic solvent system (n-heptane:acetonitrile, ratio 5:5, v/v) was used as the mobile phase. During the initial reverse phase elution, the two target compounds were obtained, which was followed by the extrusion of the stationary phase by switching the pumping of the mobile phase to the stationary phase, while maintaining the column rotation. Once the column was refilled with stationary phase, a new hydrodynamic equilibrium condition was again reached for a new separation cycle. Ten consecutive sample injections (1000 mg of algal oil, each) were performed in this way leading to the separation of DHA ethyl ester (1, 797 mg, 99% purity) and DPA n-6 ethyl ester (2, 164 mg, 97% purity) with recoveries of 99% and 92%, respectively. The process throughput of algal oil was 1.149 g/h while the efficiency per gram of two target compounds was 0.110 g/h. Environmental risk and process evaluation factors were used for evaluation of the separation process. Overall, this separation strategy may represent a useful model for the coproduction of DHA and DPA n-6 from microalgae oil under a biorefinery perspective because the potential coproduction of other valuable compounds present in both the algal oil and biomass may contribute to enhancement of the economics of the approach.

Název v anglickém jazyce

A biorefinery approach to obtain docosahexaenoic acid and docosapentaenoic acid n-6 from Schizochytrium using high performance countercurrent chromatography

Popis výsledku anglicky

Schizochytrium has arisen as a valuable alternative to fish as a source of polyunsaturated fatty acids. In the present study, Schizochytrium limacinum CO3-H biomass was processed by direct transesterification affording a transesterified algal oil, from which docosahexaenoic acid (DHA) and docosapentaenoic acid n-6 (DPA n-6) ethyl esters were isolated using high performance countercurrent chromatography (HPCCC). A multiple sequential injection HPCCC separation method was developed combining two elution modes (reverse phase and extrusion). During the reverse phase elution, the lower phase of a biphasic solvent system (n-heptane:acetonitrile, ratio 5:5, v/v) was used as the mobile phase. During the initial reverse phase elution, the two target compounds were obtained, which was followed by the extrusion of the stationary phase by switching the pumping of the mobile phase to the stationary phase, while maintaining the column rotation. Once the column was refilled with stationary phase, a new hydrodynamic equilibrium condition was again reached for a new separation cycle. Ten consecutive sample injections (1000 mg of algal oil, each) were performed in this way leading to the separation of DHA ethyl ester (1, 797 mg, 99% purity) and DPA n-6 ethyl ester (2, 164 mg, 97% purity) with recoveries of 99% and 92%, respectively. The process throughput of algal oil was 1.149 g/h while the efficiency per gram of two target compounds was 0.110 g/h. Environmental risk and process evaluation factors were used for evaluation of the separation process. Overall, this separation strategy may represent a useful model for the coproduction of DHA and DPA n-6 from microalgae oil under a biorefinery perspective because the potential coproduction of other valuable compounds present in both the algal oil and biomass may contribute to enhancement of the economics of the approach.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Algal Research-Biomass Biofuels and Bioproducts

ISSN

2211-9264

e-ISSN

2211-9264

Svazek periodika

55

Číslo periodika v rámci svazku

MAY 2021

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

12

Strana od-do

102241

Kód UT WoS článku

000642452900004

EID výsledku v databázi Scopus

2-s2.0-85101814625