Bio-production of eicosapentaenoic acid from the diatom Nanofrustulum shiloi via two-step high performance countercurrent chromatography

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F22%3A00565820" target="_blank" >RIV/61388971:_____/22:00565820 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12310/22:43905310

Výsledek na webu

<a href="https://link.springer.com/article/10.1007/s10811-022-02816-w" target="_blank" >https://link.springer.com/article/10.1007/s10811-022-02816-w</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10811-022-02816-w" target="_blank" >10.1007/s10811-022-02816-w</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Bio-production of eicosapentaenoic acid from the diatom Nanofrustulum shiloi via two-step high performance countercurrent chromatography

Popis výsledku v původním jazyce

Eicosapentaenoic acid (EPA) is an essential polyunsaturated fatty acid of nutritional and physiological relevance for humans. It is commercially produced mainly from fish oil, but new sustainable sources are required. In this study, pure EPA ethyl ester was produced from Nanofrustulum shiloi biomass by integrating two separation steps via high performance countercurrent chromatography (HPCCC). The HPCCC first step consisted of a 10 separation cycles (1200 mg sample per cycle) process, each cycle consisting of three stages comprising EPA fraction separation in reversed phase elution mode (lower polar phase used as mobile phase), with stationary phase extrusion (by switching the pumping of the mobile phase to the stationary phase, while maintaining the column rotation), and hydrodynamic equilibrium achievement inside the column. The process led to 982.1 mg of a fraction rich in EPA with a purity of 71%. To improve the purity of the EPA fraction, an HPCCC second step was performed leading to 698.97 mg of EPA ethyl ester with a purity of 99%, and a recovery of 98%. To avoid unnecessary waste of solvents, liquid phases were formulated based on a priori NMR measurement. The HPCCC process throughput was 0.936 g h(-1), while the efficiency per gram of EPA was 0.054 g h(-1). Environmental risk and process evaluation factors were used for the evaluation of the separation process. Overall, this isolation approach may represent a useful model for the efficient production of pure EPA from microalgae.

Název v anglickém jazyce

Bio-production of eicosapentaenoic acid from the diatom Nanofrustulum shiloi via two-step high performance countercurrent chromatography

Popis výsledku anglicky

Eicosapentaenoic acid (EPA) is an essential polyunsaturated fatty acid of nutritional and physiological relevance for humans. It is commercially produced mainly from fish oil, but new sustainable sources are required. In this study, pure EPA ethyl ester was produced from Nanofrustulum shiloi biomass by integrating two separation steps via high performance countercurrent chromatography (HPCCC). The HPCCC first step consisted of a 10 separation cycles (1200 mg sample per cycle) process, each cycle consisting of three stages comprising EPA fraction separation in reversed phase elution mode (lower polar phase used as mobile phase), with stationary phase extrusion (by switching the pumping of the mobile phase to the stationary phase, while maintaining the column rotation), and hydrodynamic equilibrium achievement inside the column. The process led to 982.1 mg of a fraction rich in EPA with a purity of 71%. To improve the purity of the EPA fraction, an HPCCC second step was performed leading to 698.97 mg of EPA ethyl ester with a purity of 99%, and a recovery of 98%. To avoid unnecessary waste of solvents, liquid phases were formulated based on a priori NMR measurement. The HPCCC process throughput was 0.936 g h(-1), while the efficiency per gram of EPA was 0.054 g h(-1). Environmental risk and process evaluation factors were used for the evaluation of the separation process. Overall, this isolation approach may represent a useful model for the efficient production of pure EPA from microalgae.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Applied Phycology

ISSN

0921-8971

e-ISSN

1573-5176

Svazek periodika

34

Číslo periodika v rámci svazku

SEP 1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

16

Strana od-do

2995-3010

Kód UT WoS článku

000847965100001

EID výsledku v databázi Scopus

2-s2.0-85137249326