Advanced glycosidases as ingenious biosynthetic instruments

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F21%3A00546051" target="_blank" >RIV/61388971:_____/21:00546051 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/21:10430272

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0734975021000392?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0734975021000392?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.biotechadv.2021.107733" target="_blank" >10.1016/j.biotechadv.2021.107733</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Advanced glycosidases as ingenious biosynthetic instruments

Popis výsledku v původním jazyce

Until recently, glycosidases, naturally hydrolyzing carbohydrate-active enzymes, have found few synthetic applications in industry, being primarily used for cleaving unwanted carbohydrates. With the establishment of glycosynthase and transglycosidase technology by genetic engineering, the view of glycosidases as industrial biotechnology tools has started to change. Their easy production, affordability, robustness, and substrate versatility, added to the possibility of controlling undesired side hydrolysis by enzyme engineering, have made glycosidases competitive synthetic tools. Current promising applications of engineered glycosidases include the production of well-defined chitooligomers, precious galactooligosaccharides or specialty chemicals such as glycosylated flavonoids. Other synthetic pathways leading to human milk oligosaccharides or remodeled antibodies are on the horizon. This work provides an overview of the synthetic achievements to date for glycosidases, emphasizing the latest trends and outlining possible developments in the field.

Název v anglickém jazyce

Advanced glycosidases as ingenious biosynthetic instruments

Popis výsledku anglicky

Until recently, glycosidases, naturally hydrolyzing carbohydrate-active enzymes, have found few synthetic applications in industry, being primarily used for cleaving unwanted carbohydrates. With the establishment of glycosynthase and transglycosidase technology by genetic engineering, the view of glycosidases as industrial biotechnology tools has started to change. Their easy production, affordability, robustness, and substrate versatility, added to the possibility of controlling undesired side hydrolysis by enzyme engineering, have made glycosidases competitive synthetic tools. Current promising applications of engineered glycosidases include the production of well-defined chitooligomers, precious galactooligosaccharides or specialty chemicals such as glycosylated flavonoids. Other synthetic pathways leading to human milk oligosaccharides or remodeled antibodies are on the horizon. This work provides an overview of the synthetic achievements to date for glycosidases, emphasizing the latest trends and outlining possible developments in the field.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biotechnology Advances

ISSN

0734-9750

e-ISSN

1873-1899

Svazek periodika

49

Číslo periodika v rámci svazku

JUL-AUG

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

27

Strana od-do

107733

Kód UT WoS článku

000663421300011

EID výsledku v databázi Scopus

2-s2.0-85103794506