Application Potential of Cyanide Hydratase from Exidia glandulosa: Free Cyanide Removal from Simulated Industrial Effluents

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F21%3A00549581" target="_blank" >RIV/61388971:_____/21:00549581 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68407700:21460/21:00354175

Výsledek na webu

<a href="https://www.mdpi.com/2073-4344/11/11/1410" target="_blank" >https://www.mdpi.com/2073-4344/11/11/1410</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/catal11111410" target="_blank" >10.3390/catal11111410</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Application Potential of Cyanide Hydratase from Exidia glandulosa: Free Cyanide Removal from Simulated Industrial Effluents

Popis výsledku v původním jazyce

Industries such as mining, cokemaking, (petro)chemical and electroplating produce effluents that contain free cyanide (fCN = HCN + CN-). Currently, fCN is mainly removed by (physico)chemical methods or by biotreatment with activated sludge. Cyanide hydratases (CynHs) (EC 4.2.1.66), which convert fCN to the much less toxic formamide, have been considered for a mild approach to wastewater decyanation. However, few data are available to evaluate the application potential of CynHs. In this study, we used a new CynH from Exidia glandulosa (protein KZV92691.1 designated NitEg by us), which was overproduced in Escherichia coli. The purified NitEg was highly active for fCN with 784 U/mg protein, k(cat) 927/s and k(cat)/K-M 42/s/mM. It exhibited optimal activities at pH approximately 6-9 and 40-45 & DEG,C. It was quite stable in this pH range, and retained approximately 40% activity at 37 & DEG,C after 1 day. Silver and copper ions (1 mM) decreased its activity by 30-40%. The removal of 98-100% fCN was achieved for 0.6-100 mM fCN. Moreover, thiocyanate, sulfide, ammonia or phenol added in amounts typical of industrial effluents did not significantly reduce the fCN conversion, while electroplating effluents may need to be diluted due to high fCN and metal content. The ease of preparation of NitEg, its high specific activity, robustness and long shelf life make it a promising biocatalyst for the detoxification of fCN.

Název v anglickém jazyce

Application Potential of Cyanide Hydratase from Exidia glandulosa: Free Cyanide Removal from Simulated Industrial Effluents

Popis výsledku anglicky

Industries such as mining, cokemaking, (petro)chemical and electroplating produce effluents that contain free cyanide (fCN = HCN + CN-). Currently, fCN is mainly removed by (physico)chemical methods or by biotreatment with activated sludge. Cyanide hydratases (CynHs) (EC 4.2.1.66), which convert fCN to the much less toxic formamide, have been considered for a mild approach to wastewater decyanation. However, few data are available to evaluate the application potential of CynHs. In this study, we used a new CynH from Exidia glandulosa (protein KZV92691.1 designated NitEg by us), which was overproduced in Escherichia coli. The purified NitEg was highly active for fCN with 784 U/mg protein, k(cat) 927/s and k(cat)/K-M 42/s/mM. It exhibited optimal activities at pH approximately 6-9 and 40-45 & DEG,C. It was quite stable in this pH range, and retained approximately 40% activity at 37 & DEG,C after 1 day. Silver and copper ions (1 mM) decreased its activity by 30-40%. The removal of 98-100% fCN was achieved for 0.6-100 mM fCN. Moreover, thiocyanate, sulfide, ammonia or phenol added in amounts typical of industrial effluents did not significantly reduce the fCN conversion, while electroplating effluents may need to be diluted due to high fCN and metal content. The ease of preparation of NitEg, its high specific activity, robustness and long shelf life make it a promising biocatalyst for the detoxification of fCN.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/GA18-00184S" target="_blank" >GA18-00184S: Nové proteiny "nitrilasové nadrodiny" u Basidiomycot: studium jejich aktivit a možných funkcí v biodegradaci kyanidu a nitrilů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Catalysts

ISSN

2073-4344

e-ISSN

2073-4344

Svazek periodika

11

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

16

Strana od-do

1410

Kód UT WoS článku

000723298100001

EID výsledku v databázi Scopus

2-s2.0-85119371203