Biotransformation of free cyanide to formic acid by a cyanide hydratase-formamidase cascade reaction

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F24%3A00587363" target="_blank" >RIV/61388971:_____/24:00587363 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68407700:21460/24:00382234 RIV/00216208:11310/24:10495586 RIV/60461373:22330/24:43929721

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S1359511324001041?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1359511324001041?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.procbio.2024.04.009" target="_blank" >10.1016/j.procbio.2024.04.009</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Biotransformation of free cyanide to formic acid by a cyanide hydratase-formamidase cascade reaction

Popis výsledku v původním jazyce

Free cyanide (fCN) consisting of HCN and CN is highly hazardous. Today, removal of cyanide from industrial (mining, plating, cokeplant) wastewaters largely relies on physicochemical processes followed by microbial degradation. Enzymatic processes are gaining ground but are still at a low technological stage. The cyanideconverting enzymes of interest are primarily cyanide dihydratases (CynDs, EC 3.5.5.1.), which hydrolyze HCN to formic acid and ammonia, and cyanide hydratases (CynHs, EC 4.2.1.66), which hydrate HCN to formamide. CynHs usually have much higher specific activities and a broader pH profile especially in the alkaline region compared to CynDs. However, the product of CynH, formamide, although much less toxic than fCN, still poses a significant health risk. Therefore, it is attractive to combine the CynH with an amidase that converts formamide to formic acid and ammonia. Here we demonstrate on a laboratory scale a two-step ´onepot´ detoxification of fCN (5 mM) to formic acid using recombinantly produced purified enzymes CynH from Exidia glandulosa and formamidase (EC 3.5.1.49) from Bacillus cereus . The reaction proceeds at pH 9.0-10.0, which reduces the risk of HCN escape. We also hypothesize that the cascade can be used for fCN determination after coupling an NADdependent formate dehydrogenase.

Název v anglickém jazyce

Biotransformation of free cyanide to formic acid by a cyanide hydratase-formamidase cascade reaction

Popis výsledku anglicky

Free cyanide (fCN) consisting of HCN and CN is highly hazardous. Today, removal of cyanide from industrial (mining, plating, cokeplant) wastewaters largely relies on physicochemical processes followed by microbial degradation. Enzymatic processes are gaining ground but are still at a low technological stage. The cyanideconverting enzymes of interest are primarily cyanide dihydratases (CynDs, EC 3.5.5.1.), which hydrolyze HCN to formic acid and ammonia, and cyanide hydratases (CynHs, EC 4.2.1.66), which hydrate HCN to formamide. CynHs usually have much higher specific activities and a broader pH profile especially in the alkaline region compared to CynDs. However, the product of CynH, formamide, although much less toxic than fCN, still poses a significant health risk. Therefore, it is attractive to combine the CynH with an amidase that converts formamide to formic acid and ammonia. Here we demonstrate on a laboratory scale a two-step ´onepot´ detoxification of fCN (5 mM) to formic acid using recombinantly produced purified enzymes CynH from Exidia glandulosa and formamidase (EC 3.5.1.49) from Bacillus cereus . The reaction proceeds at pH 9.0-10.0, which reduces the risk of HCN escape. We also hypothesize that the cascade can be used for fCN determination after coupling an NADdependent formate dehydrogenase.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/GA22-06785S" target="_blank" >GA22-06785S: Stanovení volného kyanidu: nové přístupy založené na enzymových kaskádách</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Process Biochemistry

ISSN

1359-5113

e-ISSN

1873-3298

Svazek periodika

142

Číslo periodika v rámci svazku

July 2024

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

6

Strana od-do

62-67

Kód UT WoS článku

001235474900001

EID výsledku v databázi Scopus

2-s2.0-85190269334