Flavonolignans from silymarin modulate antibiotic resistance and virulence in Staphylococcus aureus

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F22%3A00557462" target="_blank" >RIV/61388971:_____/22:00557462 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22330/22:43925352

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0753332222001949?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0753332222001949?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.biopha.2022.112806" target="_blank" >10.1016/j.biopha.2022.112806</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Flavonolignans from silymarin modulate antibiotic resistance and virulence in Staphylococcus aureus

Popis výsledku v původním jazyce

Antibiotic resistance is currently a serious health problem. Since the discovery of new antibiotics no longer seems to be a sufficient tool in the fight against multidrug-resistant infections, adjuvant (combination) therapy is gaining in importance as well as reducing bacterial virulence. Silymarin is a complex of flavonoids and fla-vonolignans known for its broad spectrum of biological activities, including its ability to modulate drug resis-tance in cancer. This work aimed to test eleven, optically pure silymarin flavonolignans for their ability to reverse the multidrug resistance phenotype of Staphylococcus aureus and reduce its virulence. Silybin A, 2,3-dehydrosi-lybin B, and 2,3-dehydrosilybin AB completely reversed antibiotic resistance at concentrations of 20 mu M or less. Both 2,3-dehydrosilybin B and AB decreased the antibiotic-induced gene expression of representative efflux pumps belonging to the major facilitator (MFS), multidrug and toxic compound extrusion (MATE), and ATP-binding cassette (ABC) families. 2,3-Dehydrosilybin B also inhibited ethidium bromide accumulation and efflux in a clinical isolate whose NorA and MdeA overproduction was induced by antibiotics. Most of the tested flavonolignans reduced cell-to-cell communication on a tetrahydrofuran-borate (autoinducer-2) basis, with isosilychristin leading the way followed by 2,3-dehydrosilybin A and AB, which halved communication at 10 mu M. Anhydrosilychristin was the only compound that reduced communication based on acyl-homoserine lactone (autoinducer 1), with an IC50 of 4.8 mu M. Except for isosilychristin and anhydrosilychristin, all of the fla-vonolignans inhibited S. aureus surface colonization, with 2,3-dehydrosilybin A being the most active (IC50 10.6 mu M).

Název v anglickém jazyce

Flavonolignans from silymarin modulate antibiotic resistance and virulence in Staphylococcus aureus

Popis výsledku anglicky

Antibiotic resistance is currently a serious health problem. Since the discovery of new antibiotics no longer seems to be a sufficient tool in the fight against multidrug-resistant infections, adjuvant (combination) therapy is gaining in importance as well as reducing bacterial virulence. Silymarin is a complex of flavonoids and fla-vonolignans known for its broad spectrum of biological activities, including its ability to modulate drug resis-tance in cancer. This work aimed to test eleven, optically pure silymarin flavonolignans for their ability to reverse the multidrug resistance phenotype of Staphylococcus aureus and reduce its virulence. Silybin A, 2,3-dehydrosi-lybin B, and 2,3-dehydrosilybin AB completely reversed antibiotic resistance at concentrations of 20 mu M or less. Both 2,3-dehydrosilybin B and AB decreased the antibiotic-induced gene expression of representative efflux pumps belonging to the major facilitator (MFS), multidrug and toxic compound extrusion (MATE), and ATP-binding cassette (ABC) families. 2,3-Dehydrosilybin B also inhibited ethidium bromide accumulation and efflux in a clinical isolate whose NorA and MdeA overproduction was induced by antibiotics. Most of the tested flavonolignans reduced cell-to-cell communication on a tetrahydrofuran-borate (autoinducer-2) basis, with isosilychristin leading the way followed by 2,3-dehydrosilybin A and AB, which halved communication at 10 mu M. Anhydrosilychristin was the only compound that reduced communication based on acyl-homoserine lactone (autoinducer 1), with an IC50 of 4.8 mu M. Except for isosilychristin and anhydrosilychristin, all of the fla-vonolignans inhibited S. aureus surface colonization, with 2,3-dehydrosilybin A being the most active (IC50 10.6 mu M).

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30104 - Pharmacology and pharmacy

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biomedicine & Pharmacotherapy

ISSN

0753-3322

e-ISSN

1950-6007

Svazek periodika

149

Číslo periodika v rámci svazku

May 2022

Stát vydavatele periodika

FR - Francouzská republika

Počet stran výsledku

14

Strana od-do

112806

Kód UT WoS článku

000791274500002

EID výsledku v databázi Scopus

2-s2.0-85126617330