Modeling the catarrhal stage of Bordetella pertussis upper respiratory tract infections in mice

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F22%3A00558663" target="_blank" >RIV/61388971:_____/22:00558663 - isvavai.cz</a>

Výsledek na webu

<a href="https://journals.biologists.com/dmm/article/15/5/dmm049266/275265/Modeling-the-catarrhal-stage-of-Bordetella" target="_blank" >https://journals.biologists.com/dmm/article/15/5/dmm049266/275265/Modeling-the-catarrhal-stage-of-Bordetella</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1242/dmm.049266" target="_blank" >10.1242/dmm.049266</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Modeling the catarrhal stage of Bordetella pertussis upper respiratory tract infections in mice

Popis výsledku v původním jazyce

Pertussis (whooping cough) is a highly transmissible human respiratory disease caused by Bordetella pertussis, a human-restricted pathogen. Animal models generally involve pneumonic infections induced by depositing large numbers of bacteria in the lungs of mice. These models have informed us about the molecular pathogenesis of pertussis and guided development of vaccines that successfully protect against severe disease. However, they bypass the catarrhal stage of the disease, when bacteria first colonize and initially grow in the upper respiratory tract. This is a critical and highly transmissible stage of the infection that current vaccines do not prevent. Here, we demonstrate a model system in which B. pertussis robustly and persistently infects the nasopharynx of TLR4-deficient mice, inducing localized inflammation, neutrophil recruitment and mucus production as well as persistent shedding and occasional transmission to cage mates. This novel experimental system will allow the study of the contributions of bacterial factors to colonization of and shedding from the nasopharynx, as occurs during the catarrhal stage of pertussis, and interventions that might better control the ongoing circulation of pertussis.

Název v anglickém jazyce

Modeling the catarrhal stage of Bordetella pertussis upper respiratory tract infections in mice

Popis výsledku anglicky

Pertussis (whooping cough) is a highly transmissible human respiratory disease caused by Bordetella pertussis, a human-restricted pathogen. Animal models generally involve pneumonic infections induced by depositing large numbers of bacteria in the lungs of mice. These models have informed us about the molecular pathogenesis of pertussis and guided development of vaccines that successfully protect against severe disease. However, they bypass the catarrhal stage of the disease, when bacteria first colonize and initially grow in the upper respiratory tract. This is a critical and highly transmissible stage of the infection that current vaccines do not prevent. Here, we demonstrate a model system in which B. pertussis robustly and persistently infects the nasopharynx of TLR4-deficient mice, inducing localized inflammation, neutrophil recruitment and mucus production as well as persistent shedding and occasional transmission to cage mates. This novel experimental system will allow the study of the contributions of bacterial factors to colonization of and shedding from the nasopharynx, as occurs during the catarrhal stage of pertussis, and interventions that might better control the ongoing circulation of pertussis.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/GX19-27630X" target="_blank" >GX19-27630X: Součinnost toxinů ve virulenci Bordetella pertussis</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Disease Models & Mechanisms

ISSN

1754-8403

e-ISSN

1754-8411

Svazek periodika

15

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

049266

Kód UT WoS článku

000812323200006

EID výsledku v databázi Scopus

2-s2.0-85128121790