Co-acquisition of mineral-bound iron and phosphorus by natural Trichodesmium colonies

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F23%3A00572416" target="_blank" >RIV/61388971:_____/23:00572416 - isvavai.cz</a>

Výsledek na webu

<a href="https://aslopubs.onlinelibrary.wiley.com/doi/10.1002/lno.12329" target="_blank" >https://aslopubs.onlinelibrary.wiley.com/doi/10.1002/lno.12329</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/lno.12329" target="_blank" >10.1002/lno.12329</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Co-acquisition of mineral-bound iron and phosphorus by natural Trichodesmium colonies

Popis výsledku v původním jazyce

Low iron (Fe) and phosphorus (P) ocean regions are often home to the globally important N-2-fixing cyanobacterium Trichodesmium spp., which are physiologically adapted to Fe/P co-limitation. Given Trichodesmium's eminent ability to capture particles and the common associations between Fe and P in sediments and aerosols, we hypothesized that mineral bio-dissolution by Trichodesmium spp. may enable them to co-acquire Fe and P. We present a new sensitive assay to determine P uptake from particles, utilizing P-33-labeled ferrihydrite. To validate the method, we examined single natural Trichodesmium thiebautii colonies in a high-resolution radiotracer ss-imager, identifying strong colony-mineral interactions, efficient removal of external P-33-labeled ferrihydrite, and elevated P-33 uptake in the colony core. Next, we determined bulk P uptake rates, comparing natural Red Sea colonies and P-limited Trichodesmium erythraeum cultures. Uptake rates by natural and cultured Trichodesmium were similar to P release rates from the mineral, suggesting tight coupling between dissolution and uptake. Finally, synthesizing P-ferrihydrite labeled with either P-33 or Fe-55, we probed for Fe/P co-extraction by common microbial mineral solubilization pathways. Dissolution rates of ferrihydrite were accelerated by exogenous superoxide and strong Fe-chelator and subsequently enhanced P-33 release and uptake by Trichodesmium. Our method and findings can facilitate further Fe/P co-acquisition studies and highlight the importance of biological mechanisms and microenvironments in controlling bioavailability and nutrient fluxes from particles.

Název v anglickém jazyce

Co-acquisition of mineral-bound iron and phosphorus by natural Trichodesmium colonies

Popis výsledku anglicky

Low iron (Fe) and phosphorus (P) ocean regions are often home to the globally important N-2-fixing cyanobacterium Trichodesmium spp., which are physiologically adapted to Fe/P co-limitation. Given Trichodesmium's eminent ability to capture particles and the common associations between Fe and P in sediments and aerosols, we hypothesized that mineral bio-dissolution by Trichodesmium spp. may enable them to co-acquire Fe and P. We present a new sensitive assay to determine P uptake from particles, utilizing P-33-labeled ferrihydrite. To validate the method, we examined single natural Trichodesmium thiebautii colonies in a high-resolution radiotracer ss-imager, identifying strong colony-mineral interactions, efficient removal of external P-33-labeled ferrihydrite, and elevated P-33 uptake in the colony core. Next, we determined bulk P uptake rates, comparing natural Red Sea colonies and P-limited Trichodesmium erythraeum cultures. Uptake rates by natural and cultured Trichodesmium were similar to P release rates from the mineral, suggesting tight coupling between dissolution and uptake. Finally, synthesizing P-ferrihydrite labeled with either P-33 or Fe-55, we probed for Fe/P co-extraction by common microbial mineral solubilization pathways. Dissolution rates of ferrihydrite were accelerated by exogenous superoxide and strong Fe-chelator and subsequently enhanced P-33 release and uptake by Trichodesmium. Our method and findings can facilitate further Fe/P co-acquisition studies and highlight the importance of biological mechanisms and microenvironments in controlling bioavailability and nutrient fluxes from particles.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10617 - Marine biology, freshwater biology, limnology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Limnology and Oceanography

ISSN

0024-3590

e-ISSN

1939-5590

Svazek periodika

68

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

14

Strana od-do

1064-1077

Kód UT WoS článku

000943788700001

EID výsledku v databázi Scopus

2-s2.0-85149622502