Probing Antibody Structures by Hydrogen/Deuterium Exchange Mass Spectrometry

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F23%3A00580418" target="_blank" >RIV/61388971:_____/23:00580418 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/23:10465518

Výsledek na webu

<a href="https://link.springer.com/protocol/10.1007/978-1-0716-3457-8_17" target="_blank" >https://link.springer.com/protocol/10.1007/978-1-0716-3457-8_17</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/978-1-0716-3457-8_17" target="_blank" >10.1007/978-1-0716-3457-8_17</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Probing Antibody Structures by Hydrogen/Deuterium Exchange Mass Spectrometry

Popis výsledku v původním jazyce

Hydrogen/deuterium exchange (HDX) followed by mass spectrometry detection (MS) provides a fast, reliable, and detailed solution for the assessment of a protein structure. It has been widely recognized as an indispensable tool and already approved by several regulatory agencies as a structural technique for the validation of protein biopharmaceuticals, including antibody-based drugs. Antibodies are of a key importance in life and medical sciences but considered to be challenging analytical targets because of their compact structure stabilized by disulfide bonds and due to the presence of glycosylation. Despite these difficulties, there are already numerous excellent studies describing MS-based antibody structure characterization. In this chapter, we describe a universal HDX-MS workflow. Deeper attention is paid to sample handling, optimization procedures, and feasibility stages, as these elements of the HDX experiment are crucial for obtaining reliable detailed and spatially well-resolved information.

Název v anglickém jazyce

Probing Antibody Structures by Hydrogen/Deuterium Exchange Mass Spectrometry

Popis výsledku anglicky

Hydrogen/deuterium exchange (HDX) followed by mass spectrometry detection (MS) provides a fast, reliable, and detailed solution for the assessment of a protein structure. It has been widely recognized as an indispensable tool and already approved by several regulatory agencies as a structural technique for the validation of protein biopharmaceuticals, including antibody-based drugs. Antibodies are of a key importance in life and medical sciences but considered to be challenging analytical targets because of their compact structure stabilized by disulfide bonds and due to the presence of glycosylation. Despite these difficulties, there are already numerous excellent studies describing MS-based antibody structure characterization. In this chapter, we describe a universal HDX-MS workflow. Deeper attention is paid to sample handling, optimization procedures, and feasibility stages, as these elements of the HDX experiment are crucial for obtaining reliable detailed and spatially well-resolved information.

Druh

C - Kapitola v odborné knize

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Mass Spectrometry-Based Proteomics

ISBN

978-1-0716-3456-1

Počet stran výsledku

32

Strana od-do

303-334

Počet stran knihy

336

Název nakladatele

Humana

Místo vydání

New York

Kód UT WoS kapitoly

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