Influence of standard culture conditions and effect of oleoresin from the microalga <i>Haematococcus pluvialis</i> on splenic cells from healthy Balb/c mice a pilot study

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F23%3A00583141" target="_blank" >RIV/61388971:_____/23:00583141 - isvavai.cz</a>

Výsledek na webu

<a href="https://link.springer.com/article/10.1007/s11626-023-00822-x" target="_blank" >https://link.springer.com/article/10.1007/s11626-023-00822-x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11626-023-00822-x" target="_blank" >10.1007/s11626-023-00822-x</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Influence of standard culture conditions and effect of oleoresin from the microalga <i>Haematococcus pluvialis</i> on splenic cells from healthy Balb/c mice a pilot study

Popis výsledku v původním jazyce

In this work, we used splenocytes from healthy mice to study the effects of the two most commonly used cell culture media (A, B) with different compositions of redox reagents. The incubation of cells for 24 h resulted in a significant decrease in viability and metabolic activity of splenocytes, and the negative effects of incubation in medium B were more pronounced. In standard conditions, oxidative stress in cells was manifested by reduced mitochondrial potential, and this effect correlated with the transition of 58.3% of cells to the early stage of apoptosis under reducing conditions of medium A and up to 66.1% of cells under super-reducing conditions in medium B, suggesting altered cell physiology. High levels of ROS/RNS activated transcription factor Nrf2, superoxide dismutase 1, and catalase. The higher mRNA levels of these genes were under the conditions of medium B, whose super-reducing environment in combination with the environment of conventional incubators proved to be less suitable for the cells compared to medium A. Treatment of the cells with a lower concentration (10 mu g/ml) of oleoresin obtained from the microalga H. pluvialis partially eliminated the negative effects of cultivation. Higher concentration of oleoresin (40 mu g/ml) was slightly cytotoxic, due to the significant antioxidant effect of astaxanthin, the main bioactive component of the extract, which eliminated most of the ROS/RNS acting as signalling molecules. This study shows that the standard culture conditions do not reflect the physiological in vivo cell conditions, therefore, they are not generally suitable for incubation of all cell types.

Název v anglickém jazyce

Influence of standard culture conditions and effect of oleoresin from the microalga <i>Haematococcus pluvialis</i> on splenic cells from healthy Balb/c mice a pilot study

Popis výsledku anglicky

In this work, we used splenocytes from healthy mice to study the effects of the two most commonly used cell culture media (A, B) with different compositions of redox reagents. The incubation of cells for 24 h resulted in a significant decrease in viability and metabolic activity of splenocytes, and the negative effects of incubation in medium B were more pronounced. In standard conditions, oxidative stress in cells was manifested by reduced mitochondrial potential, and this effect correlated with the transition of 58.3% of cells to the early stage of apoptosis under reducing conditions of medium A and up to 66.1% of cells under super-reducing conditions in medium B, suggesting altered cell physiology. High levels of ROS/RNS activated transcription factor Nrf2, superoxide dismutase 1, and catalase. The higher mRNA levels of these genes were under the conditions of medium B, whose super-reducing environment in combination with the environment of conventional incubators proved to be less suitable for the cells compared to medium A. Treatment of the cells with a lower concentration (10 mu g/ml) of oleoresin obtained from the microalga H. pluvialis partially eliminated the negative effects of cultivation. Higher concentration of oleoresin (40 mu g/ml) was slightly cytotoxic, due to the significant antioxidant effect of astaxanthin, the main bioactive component of the extract, which eliminated most of the ROS/RNS acting as signalling molecules. This study shows that the standard culture conditions do not reflect the physiological in vivo cell conditions, therefore, they are not generally suitable for incubation of all cell types.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10601 - Cell biology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

In Vitro - Cellular and Developmental Biology. Animal Section

ISSN

1071-2690

e-ISSN

1543-706X

Svazek periodika

59

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

14

Strana od-do

764-777

Kód UT WoS článku

001125836400002

EID výsledku v databázi Scopus

2-s2.0-85178900103